NUSANTARA BIOSCIENCE ISSN: 2087-3948 Vol. 12, No. 1, pp. 28-32 E-ISSN: 2087-3956 May 2020 DOI: 10.13057/nusbiosci/n120105 Growth and secondary metabolites content of chloroform extract of Chlorella sp. and Chlorella sorokiniana cultured on chicken broiler waste media DEVY SUSANTY 1,♥ , ADE AYU OKSARI 2 1 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Nusa Bangsa. Jl. KH. Sholeh Iskandar Km 4, Tanah Sereal, Bogor 16166, West Java, Indonesia. Tel.: +62-251-7533189, 7535605,7538760, Fax.: +62-251-8340217,  email: dvsusanty@gmail.com 2 Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Nusa Bangsa. Jl. KH. Sholeh Iskandar Km 4, Tanah Sereal, Bogor 16166, West Java, Indonesia Manuscript received: 19 November 2019. Revision accepted: 17 January 2020. Abstract. Susanty D, Oksari AA. 2020. Growth and secondary metabolites content of chloroform extract of Chlorella sp. and Chlorella sorokiniana cultured on chicken broiler waste media. Nusantara Bioscience 12: 28-32. Intensive chicken broiler farms create waste in the environment. Chicken Broiler Waste (CBW) was used as culture media for Chlorella sp. InaCC M39 and Chlorella sorokiniana InaCC M38 at various concentrations (2, 4, 6, 8, and 10%), and compared to AF6 media. The growth of Chlorella was observed every 48 hours for 10 days by counting the number of cells using a hemocytometer. The chloroform extract of Chlorella sp. and C. sorokiniana were analyzed for their phytochemical content to determine the presence of alkaloids, flavonoids, saponin, steroids, and tannin. The results showed that 2% of CBW media was the best medium for the growth of Chlorella with nitrogen (N), phosphorus (P), and potassium (K) content was 0.01% N; 0.01% P; 0.06% K respectively. The highest specific growth rate of Chlorella sp. on 2% CBW medium was on the 8 th day of culture (0.8133) with cell density of 1.6 x 10 6 cells/mL, while the highest specific growth rate of Chlorella sorokiniana was on the 6 th day (0.8907) with cell density of 2.99 x 10 7 cells/mL. The chloroform extract of the biomass of Chlorella sorokiniana contained steroid and saponin Keywords: Chlorella sp, Chlorella sorokiniana, chicken broiler waste media, the phytochemical content INTRODUCTION Waste is an unutilized organic or inorganic material, causing serious problems in the environment if not handled properly. Waste can come from various sources of the production process. Waste treatment should be done properly so that it can be utilized and continuously and reduce environmental damage. Some waste can be utilized as a microalgae culture medium in some previous studies such as liquid waste of palm oil (Mahdi et al. 2012), urban liquid waste (Djaghoubi et al. 2015), tofu liquid waste (Syaichurrozi and Jayanudin 2016; Simamora et al. 2017), and anaerobic processed swine waste (Cheunbarn and Peerapornpisal 2010). Liquid waste of palm oil was used as a culture medium for Spirulina platensis, Nannochloropsis sp, and Chlorella sp. (Mahdi et al. 2012). Spirulina platensis is one of the microalgae that has been cultured on several wastes, i.e, urban liquid waste (Djaghoubi et al. 2015), tofu liquid waste (Syaichurrozi and Jayanudin 2016; Simamora et al. 2017), and anaerobic processed swine waste (Cheunbarn and Peerapornpisal 2010). Chlorella is a microalga that can grow on the waste medium. Chlorella pyrenoidosa grew on rubber waste (Zulfarina et al. 2013) and cow manure (Azhar et al. 2017). Chlorella sp. is able to grow in brewing waste (Farooq et al. 2013), fisheries waste (Lestari et al. 2014), industrial liquid dairy waste (Garno et al. 2014), and coal polluted environment as potential organisms for coal bioremediation (Selvika et al. 2016), fisheries waste (Lestari et al. 2014), and dairy liquid industrial waste (Garno et al. 2014). Chlorella sorokiniana can grow in liquid waste and exhaust gases (Lizzul et al. 2014). Intensive broiler farmings create waste that greatly impacts the environment. West Java is the largest broiler chicken producing province in Indonesia, reaching 622,321 tons in 2017 (33.67% of contribution to national production) (BPS 2018). Broiler farming waste is chicken manure. “A chicken will produce about 33 grams of manure/tail per day” (Mulatsih, http://lppm.ipb.ac.id). In this research, chicken manure as a waste of broiler farming is utilized as the growth medium of Chlorella sp. and Chlorella sorokiniana. Febtisuharsi (2016) had compared the growth of Chlorella sp. on chicken manure media, cow manure media, and goat manure media. The result of that study was Chlorella sp. had highest cell and lipid production on chicken manure media. Broiler manure contains 2.2% Nitrogen, 1.41% Phosphor and 1.52% Kalium (Amanullah et al. 2010). The aims of the study were to determine the suitable waste concentration of chicken broiler for the growth of Chlorella sp. and Chlorella sorokiniana and to determine the secondary metabolites of the biomass of Chlorella sp. and C. sorokiniana.