Vol.:(0123456789) 1 3 Journal of Inorganic and Organometallic Polymers and Materials https://doi.org/10.1007/s10904-020-01713-4 Biological Compound Capping of Silver Nanoparticle with the Seed Extracts of Blackcumin (Nigella sativa): A Potential Antibacterial, Antidiabetic, Anti‑infammatory, and Antioxidant Sekar Vijayakumar 1,2  · Mani Divya 2  · Baskaralingam Vaseeharan 2  · Jingdi Chen 1  · Muniyandi Biruntha 3  · Luciano Paulino Silva 4  · Esteban F. Durán‑Lara 5,6  · Krishnan Shreema 7  · Shivendu Ranjan 8  · Nandita Dasgupta 8 Received: 21 June 2020 / Accepted: 13 August 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020 Abstract It is a well explored facts that need of biosynthesized nanomaterials has emerged for its several advantages in biomedical applications and the search for potential biosynthesized-nanomaterials is still ongoing. In this study an attempt was made to meet the requirement of potential biosynthesized-silver nanoparticles using the aqueous seed extract of N. sativa (Bc-AgNPs). X-ray powder difraction (XRD) confrms the biosynthesis of silver nanoparticle (supports the observation of UV–Vis spectroscopy) with face centred cubic structure. Fourier-transform infrared spectroscopy (FTIR) confrms the capping of the active ingredients of seed extract and further the average size of 34 nm was confrmed by high-resolution transmission electron microscopy (HR-TEM) and energy dispersive X-ray analysis (EDX) confrms the purity of Bc-AgNPs. Due to the capping of the active ingredients of seed extract, the Bc-AgNPs have shown signifcant antibacterial activity against Gram negative bacteria and at the lower concentration of 100 µg*ml −1 it has 89% scavenging activity against free radicals. A strong anti-infammatory (membrane stabilization) activity towards RBCs (98% at 100 µg*ml −1 ) was recorded when compared to the positive control (aceclofenac). In addition to the previous activities, a greater inhibition of carbohydrate hydrolyzing enzymes such as α-amylase, α-glucosidase and DPP-IV highlighted the antidiabetic potential of these green synthesized AgNPs. Overall, the efcient bioactivities of Bc-AgNPs advocates its future potential in biomedical products. Keywords Diabetes mellitus · Nigella sativa · Silver nanoparticles · Antioxidant · Anti-infammatory Abbreviations AgNO 3 Silver nitrate ATCC American Type Culture Collection BC Black cumin Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10904-020-01713-4) contains supplementary material, which is available to authorized users. * Sekar Vijayakumar vijaysekar05@gmail.com; sekarvijayakumar@sdu.edu.cn Jingdi Chen jdchen@sdu.edu.cn Luciano Paulino Silva luciano.paulino@embrapa.br Shivendu Ranjan shivendur@uj.ac.za; shivenduranjan@gmail.com 1 Marine College, Shandong University, Weihai 264209, People’s Republic of China 2 Nanobiosciences and Nanopharmacology Division, Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Science campus 6th Floor, Karaikudi, Tamil Nadu 630004, India 3 Vermiculture Technology Lab, Department of Animal Health and Management, Alagappa University, Science campus 6th Floor, Karaikudi, Tamil Nadu 630004, India 4 Embrapa Genetic Resources and Biotechnology, PBI, Laboratory of Nanobiotechnology (LNANO), Brasília, DF 70770-917, Brazil 5 Bio & NanoMaterials Lab| Drug Delivery and Controlled Release, Universidad de Talca, 3460000 Talca, Maule, Chile 6 Departamento de Microbiología, Facultad de Ciencias de la Salud, Universidad de Talca, 3460000 Talca, Maule, Chile 7 Department of Physics, Sri Sarada College for Women (Autonomous), Salem, Tamil Nadu 636016, India 8 Institute for Intelligent Systems, Faculty of Engineering and the Built Environment, University of Johannesburg, Johannesburg, South Africa