Original article Coronary Disease Extension Determines Mobilization of Endothelial Progenitor Cells and Cytokines After a First Myocardial Infarction With ST Elevation Manuel F. Jime ´ nez-Navarro, a, * Francisco Jesu ´s Gonza ´ lez, b Juan Caballero-Borrego, a Juan Antonio Marchal, c Noela Rodrı ´guez-Losada, a,b Esmeralda Carrillo, c Jose ´ Manuel Garcı ´a-Pinilla, a Jose ´ M. Herna ´ ndez-Garcı ´a, a Rita Pe ´ rez-Gonza ´ lez, b Gemma Ramı ´rez, d Antonia Ara ´ nega, c and Eduardo de Teresa Galva ´n a , Investigadores RECAVA (Red Tema ´ tica de Investigacio ´n Cooperativa de Enfermedades Cardiovasculares) a A ´ rea del Corazo ´n, Hospital Clı´nico Universitario Virgen de la Victoria, Ma ´laga, Spain b Fundacio ´n Instituto Mediterra ´neo para el Avance de la Biomedicina (IMABIS), Ma ´laga, Spain c Instituto de Biopatologı´a y Medicina Regenerativa (IBIMER), Universidad de Granada, Granada, Spain d Servicio de Hematologı´a, Hospital Clı´nico Universitario Virgen de la Victoria, Ma ´laga, Spain Rev Esp Cardiol. 2011;64(12):1123–1129 Article history: Received 12 May 2011 Accepted 3 July 2011 Available online 2 October 2011 Keywords: Acute myocardial infarction Multivessel acute coronary disease Single vessel acute coronary disease Vascular endothelial growth factor Hepatocyte growth factor Physiology Endothelial progenitor cells A B S T R A C T Introduction and objectives: Multivessel coronary disease is still a postinfarction prognostic marker despite new forms of reperfusion, such as primary angioplasty. The aim of this study was to determine the time sequence of various sets of endothelial progenitor cells and angiogenic cytokines (vascular endothelial growth factor, hepatocyte growth factor) according to the degree of extension of the postinfarction coronary disease. Methods: We studied the release kinetics in 32 patients admitted for a first myocardial infarction with ST elevation, grouped according to whether they had single or multivessel disease, and 26 controls. Results: The patients had a higher number of endothelial progenitor cells and angiogenic cytokines than the controls at all 3 measurements (admission, day 3, and day 7) of the following subsets: CD34, CD34+CD133+, CD34+KDR+, and CD34+CD133+KDR+CD45+(weak); this latter was higher on day 7. The levels of these cell subsets were all higher in the patients with single-vessel disease and at all 3 measurements. The vascular endothelial growth factor levels were raised during the first week and the hepatocyte growth factor showed an early peak on admission for infarction. No significant differences were seen in the cytokines according to coronary disease extension. Conclusions: Although the release kinetics of different subsets of endothelial progenitor cells in patients with a first acute myocardial infarction with ST elevation was similar in those with single vessel disease to those with multivessel disease, the number of circulating endothelial progenitor cells was greater in the patients with single vessel disease. The vascular endothelial growth factor levels were raised during the first postinfarction week and the hepatocyte growth factor were higher on admission. ß 2011 Sociedad Espan ˜ola de Cardiologı ´a. Published by Elsevier Espan ˜a, S.L. All rights reserved. La extensio ´n de la enfermedad coronaria determina la movilizacio ´n de las ce ´ lulas progenitoras endoteliales y las citocinas tras un primer infarto de miocardio con elevacio ´n del ST Palabras clave: Infarto agudo de miocardio Enfermedad coronaria aguda multivaso Enfermedad coronaria aguda monovaso Factor de crecimiento endotelial vascular Factor de crecimiento hepatocitario Fisiologı ´a Ce ´ lulas progenitoras endoteliales R E S U M E N Introduccio ´n y objetivos: La enfermedad coronaria multivaso es un importante factor prono ´ stico postinfarto a pesar de nuevas formas de reperfusio ´n como la angioplastia primaria. El objetivo del presente estudio es determinar la secuencia de variacio ´n de diferentes poblaciones de ce ´ lulas progenitoras endoteliales y factores angioge ´ nicos (factor de crecimiento endotelial vascular, factor de crecimiento hepatocitario) segu ´n el grado de extensio ´n de la enfermedad coronaria. Me ´todos: Estudiamos la cine ´ tica de liberacio ´n en 32 pacientes ingresados por un primer infarto, agrupados segu ´n tuvieran enfermedad coronaria monovaso o multivaso y 26 sujetos que constituyen el grupo control. Resultados: Los pacientes presentaban un mayor nu ´ mero de ce ´ lulas progenitoras endoteliales y citocinas angioge ´ nicas que los controles en las tres determinaciones realizadas (al ingreso, dı ´a 3 y dı ´a 7) de las siguientes subpoblaciones: CD34, CD34+CD133+, CD34+KDR+ y CD34+CD133+KDR+CD45+ (de ´ bil); este u ´ ltimo era mayor el dı ´a 7. Los valores de las tres poblaciones analizadas eran mayores en los pacientes con enfermedad coronaria monovaso en las tres determinaciones. Las cifras del factor de crecimiento endotelial vascular subı ´an durante la primera semana y las del factor de crecimiento hepatocitario * Corresponding author: Servicio de Cardiologı ´a, Hospital Clı ´nico Universitario Virgen de la Victoria, Campus de Teatinos s/n, 29010 Ma ´ laga, Spain. E-mail address: jimeneznavarro@secardiologia.es (M.F. Jime ´ nez-Navarro). 1885-5857/$ – see front matter ß 2011 Sociedad Espan ˜ola de Cardiologı ´a. Published by Elsevier Espan ˜a, S.L. All rights reserved. doi:10.1016/j.rec.2011.07.003