Research Article Journal of Analytical & Bioanalytical Techniques J ou r n a l o f A n a l y t i c a l & B i o a n a l y t i c a l T e c h n i q u e s ISSN: 2155-9872 Khansari et al., J Anal Bioanal Tech 2017, 8:2 DOI: 10.4172/2155-9872.1000351 Volume 8 • Issue 2 • 1000351 J Anal Bioanal Tech, an open access journal ISSN: 2155-9872 Keywords: Molecularly imprinted polymer; Methylphenidate; Pharmaceutical analysis; Solid-phase extraction; Afnity assay; Template polymerization Introduction Recently, there have been an increasing interest in potential applications of highly selective molecularly imprinted polymers, MIPs. Especially their applications in analysis of drugs and other compounds in biological and environmental samples. Applicability of Imprinted polymers [1-4] are in various analytical techniques, including liquid chromatography [3,5], capillary electrophoresis, capillary electrochromatography [6], solid-phase extraction [7], and ‘immunoassay’ [8], have been investigated. An inhere advantage of molecular imprinting, which has extensively been testifed by many examples above, is the possibility to synthesize sorbents with selectivity pre-determined for a particular analyte. Te fundamental step in this technique is polymerization of functional and cross-linking monomers in the presence of a templating ligand, or imprint species. Subsequent removal of the imprint molecules leaves behind ‘memory sites’, or imprints, in a solid, highly cross-linked polymer network. Te general belief holds that the functional monomers are spatially fxed in the polymer via their interaction with the imprint species during the polymerization reaction. Attention defcit hyperactivity disorder (ADHD) is a common neurobehavioral disorder in childhood, which is estimated to strike up to 10% of the general population [9,10]. Methylphenidate (MPH) is a psychostimulant drug approved primarily for the treatment of attention defcit hyperactivity disorder (ADHD) and narcolepsy [11]. Tis drug fts to the piperidine class of compounds and increases the levels of dopamine and noradrenaline in the brain through reuptake inhibition of the monoamine transporters [11]. Te main urinary metabolite is a de-esterifed product, ritalinic acid (RA), which accounts for 80% of the dosage and has a half-life of about 8 h [11]. Reviews over pharmacy databases and treatment studies have shown that the incidences of medication discontinuation or non-adherence is between 13.2% and 64% [12]. Te clinical laboratory has an important role in being able to detect MPH in serum and its metabolite RA in urine. Various analytical methods such as immunoassay [13], HPLC with UV detection [14] and more recently liquid chromatography– electrospray ionization mass spectrometry [15-17], have been proposed for measuring MPH (in serum). Tis study was meant to develop and validate a novel HPLC–SPE method with samples throughput for determinations of MPH in human serum. In these applications solid phase extraction (SPE) sample preparation procedures were used. Te method outline with a selective chromatography in combination with specifc UV detection fulflls the high quality standard required for accurate determinations in serum samples from human. Experimental Materials MPH (Figure 1) was purchased from Cerillant (Texas, USA) as 1 mg/mL solutions in methanol and RA (Figure 1) from Sigma-Aldrich (Australia). HPLC grade acetonitrile was purchased from Termo Fisher (Cambridge, UK), ammonium formate and dimethyloctylamine (DMOA) from Sigma-Aldrich (Germany), and HPLC grade methanol and reagent grade 89-91% pure formic acid from BDH (Poole, UK). *Corresponding author: Mehdi Rajabnia Khansari, Research Center, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran, E-mail: rajabniamahdi@yahoo.com Received February 11, 2017; Accepted February 23, 2017; Published February 28, 2017 Citation: Khansari MR, Shahreza S, Rezvanirad A, Nikavar A, Bikloo S, et al. (2017) Synthesis of Surface Molecularly Imprinting Polymers for Methylphenidate and its Application in Separating Methylphenidate. J Anal Bioanal Tech 8: 351. doi: 10.4172/2155-9872.1000351 Copyright: © 2017 Khansari MR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Abstract In this study, a novel approach is proposed for determination of methylphenidate in biological fuids. In this method molecularly imprinted solid-phase extraction (MISPE), as the sample extraction technique, combined with high-performance liquid chromatography (HPLC) is used. The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker, Hexane as porogen and methylphenidate as template molecule. Extraction of methylphenidate from human serum was carried out using a novel imprinted polymer as the solid-phase extraction (SPE). Various parameters affecting the extraction effciency of the polymer were evaluated. Also, the optimal conditions for the MIP cartridges were studied. The limit of detection (LOD) and limit of quantifcation (LOQ) for methylphenidate in serum samples were 1.3 and 10 ng mL -1 , respectively. The recoveries for serum samples were higher than 92%. Synthesis of Surface Molecularly Imprinting Polymers for Methylphenidate and its Application in Separating Methylphenidate Mehdi Rajabnia Khansari 1,2 , Sara Shahreza 4 , Azam Rezvanirad 1 , Amin Nikavar 2 , Shahrzad Bikloo 3 and Bahareh Sadat Yousefsani 5 1 Faculty of Pharmacy, Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2 School of Chemical Engineering, Research Center, Iran University of Science and Technology, Tehran, Iran 3 Lorstan University of Medical Sciences, Research Center, Khoramabad, Iran 4 Department of Nanobiotechnology, Tarbiat Modates University, Tehran, Iran 5 Department of Pharmacodynamy and Toxicology, School of Pharmacy, Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Open Access