Citaton: Adhiyanto C, Suwarsono EA, Hendarmin LA, et al. Detecton of the SARS-CoV-2 Omicron Variant in COVID-19 Patents from South Tangerang Using SNP-Probes S371L and K417N. J Pure Appl Microbiol. 2022;16(4):2712-2723. doi: 10.22207/JPAM.16.4.42 © The Author(s) 2022. Open Access. This artcle is distributed under the terms of the Creatve Commons Atributon 4.0 Internatonal License which permits unrestricted use, sharing, distributon, and reproducton in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creatve Commons license, and indicate if changes were made. Adhiyanto et al. | Artcle 7949 J Pure Appl Microbiol. 2022;16(4):2712-2723. doi: 10.22207/JPAM.16.4.42 Received: 04 July 2022 | Accepted: 08 October 2022 Published Online: 14 November 2022 ReSeARCH ARtiCle OPeN ACCeSS www.microbiologyjournal.org 2712 Journal of Pure and Applied Microbiology P-ISSN: 0973-7510; E-ISSN: 2581-690X *Correspondence: chrisbiomed@uinjkt.ac.id Detecton of the SARS-CoV-2 Omicron Variant in COViD-19 Patents from South tangerang Using SNP-Probes S371l and K417N Chris Adhiyanto 1 *, erike A. Suwarsono 1 , laifa A. Hendarmin 1 , Zet Harriyat 1 , Flori R. Sari 1 , Fika ekayant 1 , endah Wulandari 1 , Sit Kunarisasi 1 , Mahfuzh A. Al Farisi 2 , Devi R. Nareshwari 2 , Suryani 1 , Din F. Rochmawat 1 , Ayu latfah 1 and H-J Freisleben 3,4  1 Medical Research Laboratory, Faculty of Medicine, Syarif Hidayatullah State Islamic University, Jakarta, Indonesia. 2 Faculty of Medicine, Syarif Hidayatullah State Islamic University, Jakarta, Indonesia. 3 Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia. 4 Regional Public COVID-19 Vaccinaton Centre, Eltville am Rhein, Germany. Abstract the COViD-19 pandemic caused by the SARS-CoV-2 virus has posed a global challenge. experts from various branches of science have endeavoured to fnd solutons to control its spread, one of which has been the quick and precise detecton of the virus and its variants in patents. this study aimed to detect the presence of SARS-CoV-2, notably the rapidly spreading Omicron variant, using the spike (S)- gene target failure (SGtF) and S-gene target positve (SGtP) with the principle of the single nucleotde polymorphism (SNP)-probe test. Our descriptve experimental approach detected Omicron variants with the SNP-probe technique using samples of SARS-CoV-2 patents and controls. the probes were designed to recognize the nucleotde code of the amino acids in positons 371 and 417 of SARS-CoV-2. the existence of variants was monitored by the presence or absence of a fuorescence signal, which was translated into a sigmoidal graph using a real-tme (Rt)-PCR machine. One hundred and twelve samples that had tested positve for SARS-CoV-2 and the Omicron variant using a registered commercial kit showed a similar result to our in-house-developed SNP-probe 371 and 417 assays. the results of this study indicate that the SNP-probe we designed can be used in the detecton of the SARS-CoV-2 Omicron variant. Keywords: Coronavirus, COVID-19, Omicron Variant, RT-PCR, SARS-CoV-2, SGTF, SGTP, SNP-probe