~ 988 ~ The Pharma Innovation Journal 2018; 7(4): 988-989 ISSN (E): 2277- 7695 ISSN (P): 2349-8242 NAAS Rating: 5.03 TPI 2018; 7(4): 494-495 © 2018 TPI www.thepharmajournal.com Received: 09-01-2018 Accepted: 13-02-2018 I Ashraf Division of Clinical Veterinary Medicine, Ethics and Jurisprudence, F.V.Sc and A.H, SKUAST-K, Jammu and Kashmir, India HU Malik Division of Clinical Veterinary Medicine, Ethics and Jurisprudence, F.V.Sc and A.H, SKUAST-K, Jammu and Kashmir, India Muheet Division of Clinical Veterinary Medicine, Ethics and Jurisprudence, F.V.Sc and A.H, SKUAST-K, Jammu and Kashmir, India A Muhee Division of Clinical Veterinary Medicine, Ethics and Jurisprudence, F.V.Sc and A.H, SKUAST-K, Jammu and Kashmir, India O Shah Division of Clinical Veterinary Medicine, Ethics and Jurisprudence, F.V.Sc and A.H, SKUAST-K, Jammu and Kashmir, India U Amin Division of veterinary pathology, F.V.Sc and A.H, SKUAST-K, Jammu and Kashmir, India SA Beigh Division of Clinical Veterinary Medicine, Ethics and Jurisprudence, F.V.Sc and A.H, SKUAST-K, Jammu and Kashmir, India S Bashir Division of veterinary epidemiology F.V.Sc and A.H, SKUAST-K, Jammu and Kashmir, India N Handoo Division of veterinary surgery and radiology, F.V.Sc and A.H, SKUAST-K, Jammu and Kashmir, India H Hammadani Division of livestock production and management, MCRS, F.V.Sc and A.H, SKUAST-K, Jammu and Kashmir, India N Nazir Division of Clinical Veterinary Medicine, Ethics and Jurisprudence, F.V.Sc and A.H, SKUAST-K, Jammu and Kashmir, India A Jan Division of Clinical Veterinary Medicine, Ethics and Jurisprudence, F.V.Sc and A.H, SKUAST-K, Jammu and Kashmir, India Correspondence I Ashraf Division of Clinical Veterinary Medicine, Ethics and Jurisprudence, F.V.Sc and A.H, SKUAST-K, Jammu and Kashmir, India Longitudinal study on prevalence of sub clinical mastitis in winter season of cattle from Kashmir valley I Ashraf, HU Malik, Muheet, A Muhee, O Shah, U Amin, SA Beigh, S Bashir, N Handoo, H Hammadani, N Nazir and A Jan Abstract The longitudinal study was conducted in Kashmir division of Jammu and Kashmir to access prevalence of sub-clinical mastitis in bovines. Study was conducted from January 2017 to February 2018. A total of 810 cattle samples were collected from apparently healthy quarters of lactating crossbred cattle of Kashmir region. The diagnostic protocol employed for the study was Somatic cell count, California mastitis test (CMT), pH and Electrical conductivity. Animal wise and quarter wise incidence of SCM was 47.58% and 26.67%, respectively. The study indicates that animal with apparently healthy quarter’s harbors infection which can progress to clinical condition if animal is not treated for subclinical mastitis. So the study indicates that animals should be periodically examined for sub clinical mastitis and treated for subclinical mastitis. Keywords: sub clinical mastitis, longitudinal study cattle and prevalence Introduction Mastitis is the inflammation of parenchyma of mammary gland, characterized by physical, chemical and usually bacteriological changes in milk and pathological changes in glandular tissues [1] . It is the most dreaded disease of dairy farmers because of reduced milk production, increased treatment costs, labour, milk discarding following treatment, death and premature culling [2] . Mastitis is also associated with number of zoonotic diseases in which milk acts as a vehicle of infection. Disease is of great economic importance owing to high treatment cost, reduced milk production, culling of high producing affected animals and extra expenses for the management of affected animals. Sub-clinical mastitis is the most important diseases of dairy animals throughout the world [3] , it causes changes in the milk composition which in turn, affects the suitability of milk for processing and the quality of its products [4] . Subclinical mastitis achieves significance as it doesn’t become clinically evident and is ignored by farmers despite the fact that it affects productivity and quality of milk. Materials and Methods Animals The study included animals of both organized and unorganized dairy farms. Efforts were made to include animals of same breed, age, parity and under similar managemental conditions. Study included total of 810 lactating animals. Sampling A total of 810 milk samples were collected aseptically from apparently healthy quarters of lactating crossbred cattle of two different districts of Kashmir Valley. The relevant date including animal number, lactation number and date of calving were recorded. Laboratory examination Milk samples were subjected to Somatic cell count (SSC), California Mastitis test (CMT) (Pandit and Mehta, 1969), PH and E.C for the diagnosis of Sub clinical mastitis. The exclusion criteria and inclusion criteria The criteria employed for inclusion/exclusion in the present study was: 1. Animals with Somatic cell count (SSC) above 2 lakh were excluded from study while animals with SSC below the threshold level were included in study.