female placentas were run separately to examine sex differences. Over 30 phenotypic and functional markers were used in our CyTOF analysis. Results: The immune profile of the placenta changes considerably more throughout gestation than the peripheral blood. Interestingly, the immune profile of the endovascular compartment of the placenta is distinct from that of the peripheral blood, suggestingextensive regulationat the maternal-fetal interface. We observe significant differences in immune cell frequency and activation between the peripheral blood and endovascular compartment of the placenta. Surprisingly, we’ve identified monocyte, neutrophil, and B cell subsets in the placenta on specific embryonic days that do not appear in the peripheral blood. There are also significant differences between tissue- and endovascular-associated immune cells in the placenta. For example, the majority of basophils are tissue-associated throughout gestation, whereas the majority of neutrophils are tissue-associated until E14.5 when they primarily become endovascular-associated. Conclusion: Our results show extensive gestational immune dynamics in the placenta and regulation of peripheral blood immune cells at the maternal-fetal interface. Our deep characterization of the placenta and blood during pregnancy will serve as a resource for studies that use mouse models to examine immune perturbations, tolerance and complications during pregnancy. P2.67. CHEMOKINE AND PROLACTIN SECRETION IS MODIFIED BY ANG II AND ANG-(1-7) IN DECIDUALIZED COMPARED TO NON-DECIDUALIZED HUMAN ENDOMETRIAL STROMAL CELLS (T-HESC). Agustina Sotelo, Franco Mangone, Rosario Macchi, Cecilia Parrado, Luciana Salaverry, Brenda Almozni, Marisa Castro, Estela Rey-Rold an, Andrea Canellada. Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, C atedra de Inmunología. Instituto de Estudios de la Inmunidad Humoral Dr. Ricardo Margni (IDEHU), UBA-CONICET., Buenos Aires, Argentina Objectives: To analyze the effect of Ang II and Ang-(1-7) on the secretory profile of human endometrial stromal cells (T-HESC) decidualized in vitro. Methods: T-HESC were incubated or not (control) with dibutiryl-cAMP+- medroxyprogesterone acetate (db-cAMP+MPA), with or without estradiol (E2) for 4 or 6 days (d). The peptides (125-500 nM) were added at time 0, or 4d. The participation of Ang II receptors, Ang-(1-7) receptor Mas, and NFAT, was analyzed by preincubation with losartan, PD123319, A-779, and INCA- 6, respectively, prior to stimulation with the peptides. Prolactin (PRL), IL-8 and MCP-1 levels in 2 or 6d culture supernatants, were determined by ELISA. Differences were considered statistically significant with p <0.05. Results: Ang II nor Ang-(1-7) induced PRL secretion. Respect to control, IL- 8, but not MCP-1 levels, increased after 2d with Ang II, effect inhibited by losartan and INCA-6. Ang-(1-7) at low dose increased IL-8, but decreased MCP-1 secretion; higher doses diminished both chemokines. MPA+db- cAMP with or without E2 increased PRL and MCP-1, but not IL-8 levels, at 4-6d respect to control. In cells incubated with MPA+db-cAMP (4d), Ang II dose-dependent increased PRL and MCP-1, but not IL-8 secretion, relative to cells incubated without the peptide, effect abolished by losartan and INCA-6. Ang-(1-7) dose-depend decreased the production of PRL (reversed by A-779). Chemokine secretion showed a diminishing trend. Conclusion: Ang II or Ang-(1-7) cannot induce decidualization of T-HESC; once induced, it would be enhanced by Ang II and disadvantaged by Ang- (1-7). The peptides differentially regulates the production of chemokines in the endometrial stroma, showing opposite effects in non-decidualized compared to decidualized cells. Ang II and Ang-(1-7) would be acting through AT1R/NFAT and Mas, respectively. P2.68. A PRO- INFLAMMATORY PROFILE IN THE PLACENTA AND MATERNAL SERUM OF INFANTS WITH REDUCED FETAL GROWTH. Megan Sharps, Bernadette Baker, Helen Bischof, Tatiana Guevara, Susan Greenwood, Rebecca Jones, Alexander Heazell. University of Manchester, Manchester, United Kingdom Objectives: Placental dysfunction has been identified in cases of stillbirth, fetal growth restriction (FGR) and women with reduced fetal movements (RFM). An altered inflammatory profile is seen in FGR and RFM associated with a poor pregnancy outcome. We aimed to compare placental morphology and inflammatory profile of maternal serum and placental lysates in normally grown and growth restricted fetuses. Methods: FGR was defined as (I) IBC 3 rd (n¼14) or 5 th (n¼20) (II) a decrease of 25 centiles between third trimester estimated fetal weight and IBC in women with RFM (n¼24). Controls (20 th -80 th IBC) were matched for gestational age, fetal sex and maternal characteristics. Cyto- kines and DAMPs were measured (ELISA) in placental lysates and maternal serum. Placental vascularity (CD31), syncytial nuclear aggregates (SNAs) and immune cells (CD45 and CD163) was assessed by immunohistochemistry. Results: There were significantly more CD163 + macrophages and CD45 + cells in infants with a decreased growth rate (p0.02) but not in 3 rd IBC. There was no change in vascularity but there was an increase in SNAs in fetuses with a decreased growth rate (p0.02). Maternal serum and placental lysates from infants with a decrease growth rate had a pro-in- flammatory profile (Table 1). Table 1. Change in cytokine and DAMP levels in placental lysates and maternal serum from FGR groups compared to normally grown infants. Mann-Whitney or Kruskal- Wallis as appropriate. Conclusion: The pro-inflammatory profile in both maternal serum and placental lysates of infants with a decreased growth rate suggests that inflammation is linked with reduced fetal growth in late pregnancy. P2.69. LPS FROM PORPHYROMONAS GINGIVALIS IMPAIRS TROPHOBLAST CELL FUNCTION AND IMMUNE-TROPHOBLAST INTERACTION Vanesa Hauk, Guillermina Calo, María Rom an Mu~ noz, Daiana Vota, F atima Merech, Daniel Paparini, Rosanna Ramhorst, Claudia P erez Leir os. Laboratorio de Inmunofarmacología. Instituto de Química Biol ogica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), CONICET- Universidad de Buenos Aires, Buenos Aires, Argentina Objectives: Porphyromonas gingivalis (Pg), an important pathogen of periodontal disease, has been implicated in adverse pregnancy outcome although the mechanisms involved are still unclear. Lipo- polysaccharide from Porphyromonas gingivalis (Pg-LPS), the main viru- lence factor of Pg, differs from other bacterial LPS in its structure and function: due to the presence of lipoproteins, Pg-LPS activates not only TLR4 but also TLR2 mediated pathways. The aim of this study was to examine the effect of Pg-LPS on trophoblast cell function and tropho- blast-neutrophil interaction and to explore TLR4/TLR2 mediated mechanisms. Methods: Swan-71 human trophoblastic cell line was treated with Pg- LPS (10ng/ml). Cytokine and chemokine expression was evaluated by RTqPCR, glucose uptake by flow cytometry using the fluorescent analogue 2-NBDG and cell invasion assessed in Matrigel-covered transwells Peripheral blood neutrophils were purified from healthy donors and cultured with conditioned media of trophoblast cells (TbCM) treated or not with LPS (PgLPS-CM); apoptosis was determined Abstracts / Placenta 83 (2019) e1ee118 e88