Available online at www.pelagiaresearchlibrary.com Pelagia Research Library Advances in Applied Science Research, 2012, 3 (1):591-598 ISSN: 0976-8610 CODEN (USA): AASRFC 591 Pelagia Research Library Studies on nutritional requirements of Pseudomonas aeruginosa for lipase production T. Kathiravan 1 , J. Marykala, A. Sundaramanickam, S. Kumaresan and T. Balasubramanian 1 Centre of Advance Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, India 2 Department of Microbiology, Jaya Arts and Science College, Thiruninravur Chennai, India _________________________________________________________________________________________ ABSTRACT A nutritional requirement for the lipase production by Pseudomonas aeruginosa strain was investigated. The bacterial strain was isolated from municipal sewage and it was culture with different nutritional composition. The maximum lipase activity was obtained with maltose as the sole carbon source 6.5 Uml -1 , followed by lactose 4.6Uml - 1 . The various salts used the best results were observed with calcium chloride 4.6 Uml -1 as and sodium chloride an inorganic source. Among the various commercial grade oil highest activity was observed with coconut oil 8.1 Uml -1 and gingili oil 6.8 Uml -1 . Amino acids are also play a significant role for the lipase production. The maximum lipase activity was observed with glycine 5.1Uml -1 followed by tryptophan 4.6Uml -1 as a supplement. The organism grows optimally in the temperature of 40 o C and pH 8. The strain isolated from the municipal sewage is industrially important from in terms of ability. These findings will be helpful in prospect for increase the production of industrial enzyme. Keywords: Lipase; Pseudomonas aeruginosa, optimization, lipase activity. __________________________________________________________________________________________ INTRODUCTION Enzymes are biological catalysts that allow chemical reactions to occur in living organisms at ambient conditions. Microbial enzymes are often more useful than enzymes derived from plants or animals because of the great variety of catalytic activities such as the possibility of high yields, ease of genetic manipulation, regular supply due to absence of seasonal fluctuations and rapid growth of microorganisms with inexpensive media [Dharmsthiti S and Kuhasuntisuk,1998, Kojima, Y. et al.,1994]. Among microbial enzymes the lipase has been studied extensively [Karl-Erich J and Reetz, 1998]. Lipases (EC 3.1.1.3) comprise a group of enzymes which catalyze the hydrolysis of triglycerols. In the recent years, the interest on lipase has grown significantly. The development of technologies using lipase for the synthesis of novel compounds result in expanding into new areas and increase in number of industrial applications [Wiseman, A 1995]. Lipases are extremely versatile enzymes, showing many interesting properties in industrial applications. Microbial lipases have high demand due to their specificity of reaction, stereo specificity and less energy consumption than conventional methods. Microbial lipases have wide application in the processing of food, leather, domestic, industrial wastes, cosmetic, detergents and pharmaceutical industries [ Houde et al., 2004, Wang et al., 1995] its mainly due to their ability to catalyze esterification, interesterificcation and transesterification reactions in aqueous media [Khyami-Horani H,1996]. Extracellular lipases have been produced from several microorganisms, such as fungi, yeast, and bacteria beside from plants and animals. Many commercial lipases produced from Pseudomonas cepacia, Pseudomonas alcaligenes, Pseudomonas mendocina Burkholderia studied by [Johnvesly and Naik 2001, Krieg and Holt, 1984].