Int.J.Curr.Microbiol.App.Sci (2020) 9(10): 732-737 732 Original Research Article https://doi.org/10.20546/ijcmas.2020.910.088 Safety and Cross Protective Efficacy of Bacteriophage Lysate against Pasteurella multocida Infection in Mice R. Durairajan * , Harshit Verma, Awadwsh Prajapati, Mohammmed Abbas, Mayank Rawat and Rishender Verma Division of Biological Standardization, Indian Veterinary Research Institute (IVRI), Izatnagar, Dist. Bareilly, Uttar Pradesh, India *Corresponding author ABSTRACT Introduction Haemorrhagic septicaemia (HS), the fatal septic-shock of cattle and buffaloes, and Fowl Cholera (FC) of poultry are the two ‘Pasteurelloses’ against which safe and effective bacterins are currently available commercially, and used. Conventional inactivated bacterins against HS and FC lack the ability to induce an across- serotype or serogroup response. HS vaccines containing killed whole cells of P 52 (B: 2), or any of the recognized vaccine strain, along with an adjuvant, fail to induce a cross- protective response against serotypes of A (Samanta and Rawat 2005). Hence this study was made to determine bacteriophages, and phage lysate products have been identified as suitable alternative vaccine. Also exploits cost protective solid immunity to control the different serotypes of Pasteurella multocida infection in poultry and livestock. International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 10 (2020) Journal homepage: http://www.ijcmas.com In this study established the methodology to isolate highly virulent bacteriophage and assessed the ability of this phage lysate to induce protection against wild-type challenge in mice and the natural host chickens. Subcutaneous administration of phage lysate to mice stimulated significant protection against Pasteurella multocida P 52 and A1 serotypes. But, significantly phage lysate was able to induce protection through subcutaneous route against challenge with wild type Pasteurella multocida A1 serotype in chicken but not through intranasal administration of phage lysates Keywords Bacteriophage, Lytic, P. multocida type A, Isolation, Therapeutic application Accepted: 07 September 2020 Available Online: 10 October 2020 Article Info