Himani Punia and Jayanti Tokas * Department of Biochemistry, CCS Haryana Agricultural University, Hisar, Haryana, India. Keywords: Buttermilk Human health Lipid Milk fat globule membrane Phospholipids Protein Sphingomyelin HIGHLIGHTS • Milk fat globule membrane is a complex biological membrane of lipid-protein, surrounded by the minute fat globules. • MFGM holds a great potential as nutraceutical with health beneficial properties. • Functionally important bioactivities associated with milk fat globule membrane protein include immune- stimulating, antimicrobial and antiviral properties. • MFGM proteins plays a role in a protection against colon cancer and gastrointestinal stress. * Corresponding Author: Email: jiyandri@gmail.com (J. Tokas) http://orcid.org/0000-0003-3013-3157 ABSTRACT Over the recent couple of years, milk fat globule membrane perceived a considerable attention due to its complexity in structure and health beneficial properties. This chapter describes the complex structure of the milk fat globule membrane as potential ingredient of dairy products as nutraceutical. The structure and function of milk fat globule membrane proteins are significant markers of the dietary nature of milk which might be ensnared in an assortment of medical advantages. Utilization of MFGM as nutraceutical depends upon its chemical composition, modifications introduced during processing and individual specific prepared food products. The MFGM and its associated proteins are involved in energy production, signal transduction, metabolic process regulation, cell to cell communication, and boost up immune system. This cross examination gives more bits of knowledge into the dynamic organization of human MFGM proteins, which thus will improve our comprehension of the physiological noteworthiness of MFGM proteins. We present the summary of the advances of research and functions of membrane and its associated proteins that are relevant to health and wellness. Milk fat globule membrane (MFGM) has attained a greater consideration as a potential source of nutraceutical with regards to its lipid-soluble vitamins, phospholipids and essential fatty acids. Milk Fat Globule Membrane (MFGM):An Ingredient of Dairy Products as Nutraceutical Review Article Trends in Peptide and Protein Sciences Volume 4 (2019): e7 Article history: Received: 16 August 2019 Accepted: 7 September 2019 DOI: http://dx.doi.org/10.22037/tpps.v4i0.26745 Introduction Since 17 th century observations, fat in milk composed mainly of triglycerides, exists as minute globules (0.1 to 15 μm). About 99% of cow milk lipids are composed of these minute droplets called as milk fat globule, originates at the basal region of secretory cells. These lipid droplets are enclosed by a special lipid bilayer membrane and associated proteins, designated the milk fat globule membrane (MFGM). Milk fat globule membrane is a complex biological membrane of lipid- protein, surrounded by the minute fat globules mainly comprised of polar lipids and specific membrane fat globule proteins (Phan et al., 2016a; He et al., 2017; Li et al., 2018). Milk fat globule membrane fractions contains amphiphilic properties which serve as good natural