[O-1195] EXPLORATORY STUDY FOR THE APPLICATION OF PHOTODYNAMIC DIAGNOSIS TO BILIARY PANCREATIC SURGERY Takehiro Noji, Hajime Narasaki, Toshihiro Kushibiki, Kimitaka Tanaka, Yoshitsugu Nakanishi, Toshimichi Asano, Toru Nakamura, Takahiro Tsuchikawa, Keisuke Okamura and Satoshi Hirano Department of Gastroenterological surgery II, Hokkaido University, Japan Introduction: Photodynamic diagnosis (PDD) is one of the candidates for navigation surgery in HPB malignancies. The purpose of this presentation is to show our exploratory study for the application of 5-ALA-PDD and ICG-PDD to biliary pancreatic surgery. Method: Two studies were enrolled. Result: Study 1: 5-ALA-PDD on an animal model. The purpose of this model study was to determine if 5-ALA- PDD could detect lesions in murine models of biliary- pancreatic cancers. Methods: A murine peritoneal dissem- inated nodule model was established by intraperitoneal injection of TFK-1 and PK-1 cells. Results: In the murine model of peritoneal dissemination, micro-disseminated nodules (< 1 mm) that could not be detected under white light were clearly visible using 5-ALA-PDD. Study 2: ICG- PDD. A) ICG-PDD on the hepatoduodenal ligament to the para-aortic area. The purpose of this study was to investi- gate whether ICG-PDD could demonstrate LF/lymph nodes (LN) of biliary or pancreatic tumor. Methods: 43 patients with HPB malignancies were enrolled in this study. After 5 mg/ml of ICG (0.5 ml) was injected into the Calot’s tri- angle, LF/LN were observed. Results: LF/LN were detec- ted in 29 out of 43 patients, on the hepatoduodenal ligaments or in the para-aortic area. Fluorescent signals were recorded in the LN along the bile duct and the pos- terior surface of the head of the pancreas. We also detected PAN fluorescent signals on the right side of the aorta, around the left renal vein. B) PDD for pancreatic neuro- endocrine tumor (pNET). Methods: Two cases of pNET (pancreatic body and head) were evaluated. After laparot- omy, 5 mg/ml of ICG (0.5 ml) was injected; LF and LN were then observed. Results: ICG accumulated in LN 8a on the pancreatic body pNET, and LN14d were also detected on the pancreatic head tumor. Conclusion: In conclusion, ICG-PDD can be applicable for navigation surgery to identify areas of lymphadenectomy in HPB malignancies. [O-1197] GOLD NANOPARTICLES INHIBIT COLON CANCER LIVER METASTASIS THROUGH REGULATION OF THE TGF- b PATHWAY Binhao Zhang and Chao Wang Department of Surgery-liver, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, China Introduction: The application of gold nanoparticles (AuNPs) to the management of cancer is currently the new breakthrough in cancer research. It is non-toxicity, easy to detect and with high sensitivity. However, the role of AuNPs on colon cancer liver metastasis (CRCLM) and the mechanism by which AuNPs regulate CRCLM are unclear. AuNPs combine with molecules rich in sulfenyl and amino, thus modify the structure and function of proteins containing cysteine and lysine. TGF- b is a homodimer structured by disulfide linkage. TGF-b signaling pathway plays an important role in the development and progression of colon cancer. Therefore, AuNPs might inhibit CRCLM through modifying the structure and function of TGF-b. Method: We established two TGF-b inducible luciferase colon cancer cell line and orthotopic colon cancer and liver metastasis animal models. We also overexpressed or knockdown Smad4, an important transduction factor of the TGF-b signaling pathway, to explore the mechanism by which AuNPs regulate CRCLM. Result: The following main results were harvested in this research. 1) AuNPs inhibited TGF-b secretion of colon cancer, and downregulated the downstream reporter genes of TGF-b signaling pathway. 2) AuNPs inhibited cell proliferation, migration and invasion in vitro, and reduced tumor growth and liver metastasis in vivo. 3) AuNPs inhibited the phosphorylation of ERK, P38 MAPK and AKT, leading to down-regulation of VEGF secretion and Vimentin while up-regulation of E-Cadherin. Therefore, angiogenesis and EMT was inhibited, and liver metastasis was prevented. 4) When Smad4 was knockdown, the colon cancer cell line were significantly more sensitive to AuNPs treatment, and the non-Smad pathway were even more significantly inhibited with AuNPs treatment. Conclusion: These results provided evidences that AuNPs inhibit CRCLM by blocking EMT through inhibiting the TGF-b/ ERK pathway and by reducing angiogenesis through down-regulating the TGF-b/P38 MAPK or PI3K/ AKT pathway. Loss of Smad4 sensitized colon cancer to AuNPs. [O-1462] PREOPERATIVE PORTAL VEIN LIGATION MEETS UNMET LIVER REGENERATION FOLLOWING HEPATO-PANCREATECTOMY THROUGH AUGMENTED AUTOPHAGY- TRASH TO CASH Chun-Yi Tsai 1 , Yi-Hsiu Chung 2 , Kun-Ju Lin 2 and Ta-Sen Yeh 1 1 Department of Surgery, and 2 Department of Nuclear Medicine and Center for Advanced Molecular Imaging and Translation, Chang Gung Memorial Hospital, Taiwan Introduction: HPD (hepatopancreaticoduodenectomy) is only hope for cure for horizontally- spreading perihilar bile duct cancer, however being weighed against life-threat- ening liver failure. Portal vein embolization (PVE) is increasingly employed to safeguard future liver remnant. We aim to elucidate 1) to what extent of liver regeneration compromised by HPD and 2) is PVE a solution and how? Method: Sprague-Dawley rats were allocated into 3 groups: Hx (70% hepatectomy); Hx+ Px (Hx plus 50% pancreatectomy); PVL +Hx+Px (portal vein ligation 3 days followed by Hx+Px). Restituted liver mass, Ki-67 index, HPB 2019, 21 (S2), S224eS236 S234 A-PHPBA 2019: Oral Abstracts