American Journal of Chemical and Biochemical Engineering 2017; 1(1): 1-6 http://www.sciencepublishinggroup.com/j/ajcbe doi: 10.11648/j.ajcbe.20170101.11 Enhancement of Biogas Production by Cellulytic Bacteria from Bagasse Using Methanogenesis Md. Abdur Rashid Mia, Md. Rasel Molla, Tanzina Sayed, Md. Moksadul Amin, Tanzima Yeasmin, Md. Belal Uddin * Department of Biochemistry & Molecular Biology, Rajshahi University, Rajshahi, Bangladesh Email address: * Corresponding author To cite this article: Md. Abdur Rashid Mia, Md. Rasel Molla, Tanzina Sayed, Md. Moksadul Amin, Tanzima Yeasmin, Md. Belal Uddin. Enhancement of Biogas Production by Cellulytic Bacteria from Bagasse Using Methanogenesis. American Journal of Chemical and Biochemical Engineering. Vol. 1, No. 1, 2017, pp. 1-6. doi: 10.11648/j.ajcbe.20170101.11 Received: July 13, 2016; Accepted: October 29, 2016; Published: November 23, 2016 Abstract: Energy is essential to meet the basic needs of life, to increase amenities and modernization. The main sources of energy that are met our energy demands are mineral oil, coal, natural gas and firewood. These conventional energy sources are being depleted day by day. So renewable, alternative and effective energy sources should be explored for our country as well as whole world. The production of biogas serves as an alternative energy source. The main objective of our research work was enhancement of biogas production by cellulytic bacteria from bagasse using methanogens. Five liters capacity glass reactors were used. Five sets of batch modes anaerobic digesters were used under laboratory condition. Bagasse was used as feed materials. Bagasse is the by-product of sugar mill and it was used as raw materials for paper production in our paper mills. Now it is discarded and creates a problem of sugar mills to use and manage bagasse. The raw materials were diluted with supply water in the ratio of 1 to 9 for bagasse. The characteristics of the influent slurry in term of Total Solid (T.S)%, Volatile Solid (V.S)%, P H and temperature ranges were determined every 7 days intervals for bagasse. The percentage of methane of biogas obtained from bagasse was 80%. The S 1 strain (Monococcus sp.) and S 3 strain (Streptococcus sp.) of cellulytic bacteria produced 3.45×10 -3 (m 3 /day/kg feedstock) biogas and 3.85×10 -3 (m 3 /day/kg feedstock) biogas at 22 th day respectively whereas control produced 2.85×10 -3 (m 3 /day/kg feedstock) biogas at 34 th day by using bagasse as feedstock. The results clearly demonstrated that the rate of biogas production was increased by S 1 strain and S 3 strain of cellulytic bacteria. The cumulative biogas production was found 54.20×10 -3 m 3 , 66.21×10 -3 m 3 and 61.59×10 -3 m 3 for control, S 1 strain and S 3 strain of cellulytic bacteria, respectively. In conclusion, results obtained from the present research work can be used to design biogas reactor in the field conditions to operate batch and semi-continuous mode for disposal management of sugar mills and thereby contribute a lot of in our fuel and fertilizer sectors. Keywords: Biogas, Alternative Energy, Bagasse, Cellulytic Bacteria, Biogas Plant 1. Introduction As the time being passed the population of the world is increasing gradually and the requirement of energy is also increasing. The increasing energy requirement is due to fulfill basic needs of the population of developing countries and to increase amenities and modernization of the population of the developed countries. But the main energy sources that are met energy demands are mineral oils, coal and natural gas. So it is suspected that this deposited natural energy may be depleted one day. Hence now a day a burning question has been arisen to find out a renewable alternative energy source. An anaerobic digestion process or biogas production has already been identified as an alternative energy source. The fraction of renewable energy forms for energy supply is constantly increasing since fossil fuels are running short and energy production from fossil fuels brings about emissions of the greenhouse gas carbon dioxide which has implications on the climate and environments. In this context the production of biogas by means of fermentation of biomass becomes more and more important because biogas is regarded as a clean, renewable and environmentally compatible energy