IOSR Journal Of Environmental Science, Toxicology And Food Technology (IOSR-JESTFT) e-ISSN: 2319-2402,p- ISSN: 2319-2399. Volume 5, Issue 4 (Jul. - Aug. 2013), PP 49-56 www.Iosrjournals.Org www.iosrjournals.org 49 | Page Production Of Bioethanol From Corncob And Sugarcane Bagasse With Hydrolysis Process Using Aspergillus niger and Trichoderma viride Dimas Sultan Syawala 1 , Tatik Wardiyati 2 , Moch. Dawam Maghfoer 2 1 Graduate School of Plant Science, Faculty of Agriculture, Brawijaya University, Jl. Veteran Malang 65145, East Java, Indonesia. 2 Faculties of Agriculture, Brawijaya University, Jl. Veteran Malang 65145, East Java, Indonesia. Abstrack : Bioethanol is an alternative fuels derived from biomass that is more environmentally friendly and renewable. Corncob and sugarcane bagasse are a potential agricultural wastes used as raw material for bioethanol because it has a high carbohydrate content and abundant availability. The efforts to increase bioethanol production performed by enzymatic hydrolysis method using fungus cultures of Aspergillus niger and Trichoderma viride. This study aims to determine the best combination of the addition of A. niger and T. viride cultures on corncob and sugarcane bagasse raw materials which produces optimal bioethanol production with high substrate efficiency and low residual sugars content. The results showed that the use of corncob raw materials with the addition of Aspergillus niger culture is the best treatment combination that produces ethanol at 282,3 ml/kg with 90,35% substrate efficiency. While the bagasse raw material, the addition of Trichoderma viride culture is the best treatment combination that produces ethanol at 299,2 ml/kg with 90,95% substrate efficiency. Keyword: Bioethanol, hydrolysis, Aspergillus niger, Trichoderma viride, fermentation I. Introduction The use of fossil fuels contributes for a very large air pollution, which transportation sector contributes about 80% of CO 2 emissions in atmoster. Bioethanol (C 2 H 5 OH) is an ethanol produced from biomass or natural raw materials through fermentation of sugar with the help of microorganisms, such as yeast. Bioethanol is a fuel that is more environmentally friendly and renewable. Some species of plants have been investigated for bioethanol production, such as cassava, sugarcane, corn and waste products of agriculture (Muthuvelayudham and Viruthagiri, 2007). Potential agricultural waste used as bioethanol raw materials are corncob and sugarcane bagasse. Corncob has a high carbohydrate content and is able to produce ethanol at 0,358 liter/kg dry mass. While bagasse can produce ethanol at 0,279 liter/kg dry mass (Kuhad and Singh, 1993). Bioethanol production process generally consists of four steps, namely the process of material preparation or pretreatment, hydrolysis, fermentation and distillation. Hydrolysis process is an important step because it determines the amount of glucose produced as a substrate to be fermented into ethanol. Hydrolysis process can be carried out with an acid catalyst, the catalyst combination of acid and enzymes, as well as the catalyst enzyme and enzyme (Muthuvelayudham and Viruthagiri, 2007; Patel et al., 2007). Bioethanol production is still not optimal. One is because the saccharification process only lasts for destruction by the heating process, so that the amount of sugars produced is not optimal. The efforts to increase the production of bioethanol can be done by using a cellulolytic fungus culture in the process of hydrolysis of lignocellulose by enzymatic hydrolysis method. Lignocellulose degrading enzymes are cellulase and hemicellulase. Some groups are often utilized cellulolytic fungus in lignocellulose degradation are Aspergillus niger and Trichoderma viride (Prasad et al., 2009; Tribak et al., 2002). Both of this fungus consist of three classes of cellulase enzymes that endoglucanases, celllobiohydrolases and β-glucosidases (Juwaied et al., 2011). Cellulase enzyme activity produced by A. niger and T. viride is higher compared with the other fungus, namely A. nidulans, A. terreus, A. wentti, T. reesei, T. koninigii and T. linqnorum (Juwaied et al., 2011). The purpose of this research is to determine the best combination of the addition of culture Aspergillus niger and Trichoderma viride on corncob and sugarcane bagasse raw materials which produces optimal bioethanol production and high substrate efficiency with low residual sugar content. II. Materials And Methods 2.1Tools and materials Materials that used in the bioethanol production are corncob, sugarcane bagasse, fungus cultures (Aspergillus niger and Trichoderma viride) and yeast (Saccharomyces cerevisiae). Chemical materials in the