CONCLUSIONS: Our surgical standardization seem to be effective in lowering the risks of AL, reoperation, and SSI after colorectal cancer surgery. Systematic Implementation of a Colon Bundle Significantly Decreases Surgical Site Infections Fiona M Gaunay, MD, Titilayo O Adegboyega, MD, Cesar E Sanz, MD, Marco Berrones, David E Rivadeneira, MD, MBA, Jason Lei, MD Huntington Hospital, Northwell Health System, Huntington, NY INTRODUCTION: Surgical site infections (SSIs) represent signifi- cant morbidity and financial implications after colon surgery. The objective of this prospective study is to compare clinical out- comes pre- and post-implementation of a dedicated colon surgery bundle to reduce SSIs in our health system. METHODS: A prospective study was conducted in which a dedi- cated colon surgery bundle and interdisciplinary team for its imple- mentation was established. The twenty-five components were divided into pre-hospital and pre-, intra-, and postoperative mea- sures. These included standardized mechanical and antibiotic bowel preparation, skin cleansing, alcohol-based skin preparation, maintenance of normothermia, antimicrobial prophylaxis, optimal tissue oxygenation, glucose control, a clean standardized fascial closure process, and negative pressure wound therapy. Specific enhanced preoperative patient education was provided. Consecu- tive patients who underwent a colorectal procedure between January 2015 and January 2016 were included. SSIs were recorded and subdivided by wound class. RESULTS: Implementation of the colon bundle led to a signifi- cant decrease in SSIs 7% (11/198) vs 15% (26/175) (p <0.05) when compared to the year prior. Additionally, SSIs observed in clean-contaminated and contaminated procedures decreased from 34.6% to 14.3% and 38.5% to 14.3%, respectively (p<0.05%). CONCLUSIONS: We demonstrate that the implementation of a specific colon bundle resulted in a 54% decrease in post-operative SSIs. The greatest reduction of SSIs was seen in wound classes II and III. This approach to incorporating an advanced surgery bundle for colon and rectal procedures can provide an effective strategy to reduce SSIs. Gut Microbiota in Symptomatic Uncomplicated Diverticular Disease Charlotte L Kvasnovsky, MD, Lex Leong, PhD, Guy CJ Abell, PhD, Savvas Papagrigoriadis, MD, FRCS, Kenneth D Bruce, PhD, Geraint B Rogers, PhD King’s College London, London, United Kingdom; University of Maryland Medical Center, Baltimore, MD INTRODUCTION: There is growing consensus that symptomatic uncomplicated diverticular disease is a chronic inflammatory con- dition, and that alterations in the gut microbiota may contribute to its pathogenesis. METHODS: Stool samples were obtained and frozen from 30 indi- viduals with symptomatic uncomplicated diverticular disease. DNA was extracted and 16S rRNA gene sequencing was performed. RESULTS: Assessment of the fecal microbiota composition at the bacterial phylum level showed a predominance of Firmicutes (me- dian relative abundance, 66.0%). Bloating scores and levels of fecal calprotectin were each found to be related to the relative abundance of bacterial taxa. No significant difference was observed in any alpha diversity measure, nor between the relative abundance of detected taxa, in the 2 patient groups. However, Lactobacillus abundance and inflammatory activity correlated significantly. With the exception of a difference in relative abundance of Firmi- cutes (Mann-Whitney U-test; p  0.05), the relative bacterial abundances identified were not significantly different between PD and NPD patients at the phylum level. CONCLUSIONS: In patients with symptomatic uncomplicated diverticular disease, those patients with and without a prior diver- ticulitis episode did not differ significantly. Patients’ symptoms correlate with altered bacterial populations, intestinal inflammation correlates with Lactobacillus abundance. Coloration of Calpoprotectin and Prealbumin Serum Levels in Patients with Colorectal Cancer Marianthi Chatzikonstantinou, Georgios Chatzikonstantinou, MD, PhD, FACS, Panagiotis Konstantopoulos, Spyros Stergiopoulos, Despina Perrea, Paraskevakou Georgia, Dimitroulis Dimitris National & Kapodistrian University of Athens, Athens, Greece INTRODUCTION: Under inflammatory conditions, calprotectin is released from neutrophils as a heterodimeric complex composed of S100A8 and S100A9 proteins. The S100A8/A9 complex has the capacity to induce apoptosis by direct binding to MM46 cells via zinc regulation. Simultaneously, the acute phase response (APR) is initiated, raising the number of cytokines which trigger the syn- thesis of the positive acute phase reactants, including CRP, and down-regulate plasma concentrations of albumin, transferrin, and prealbumin, a tetramer composed of 4 identical subunits that binds to triiodothyronine (T3) and holo-retinol-binding protein (RBP). METHODS: To examine the coloration between, calpoprotecin and prealbumin serum concentrations, and colorectal cancer, serum samples from 80 healthy subjects (control) and 80 colorectal cancer patients with 0.0 CRP levels were collected. The samples were analyzed using commercially available enzyme-linked immu- nosorbent assay (ELISA) kits. RESULTS: In the healthy subjects median concentrations were: preabumine11.10 ng/mL and calprotectin e4.81ng/mL. Whereas, in patients with colorectal cancer, median concentration were: pre- abumin 3.8 ng/mL and calprotectin 13.6 ng/mL. CONCLUSIONS: The results regarding pre-albumin are in accor- dance to the pertinent literature. Regarding calpoprotecin the increased number of false negative results (low concentration levels) Vol. 225, No. 4S2, October 2017 Scientific Poster Presentations: 2017 Clinical Congress e71