Australian Journal of Basic and Applied Sciences, 3(4): 4376-4383, 2009 ISSN 1991-8178 Corresponding Author: Ayman Hassan, CAPL, 7, Nadi El-Said St, Dokki, 12618, Giza, Egypt. 4376 Determination of Chlorinated Organic Pesticide Residues in Water, Sediments, and Fish from Private Fish Farms at Abbassa and Sahl Al-Husainia, Shakia Governorate Hussein El-Mekkawi, Magdy Diab, Mohamed Zaki, Ayman Hassan 1 1 1 2 The National Center of Social and Criminological Research, Giza, Egypt. 1 The Agricultural Research Center (The Central Agricultural Pesticide Laboratory), Giza, Egypt. 2 Abstract: Monitoring of chlorinated organic pesticides represents a great task due to their long lasting effect in the environment. A great deal of domestic, agricultural, and industrial wastes are thrown in Bahr El-Bakar drainage canal. This drain finally pours its water in Manzala Lake, north of Egypt, where extensive fishing activities exist. The toxicity of these pesticides on human and other living things encourages scientists to examine their levels in the environment elements (water and sediment). Fish is considered an important source of food to a great portion of populations. As theses aquatic organisms accumulates toxic substances in their organs, determination of these toxic pesticides in fish samples is largely important. As large as a total of 400 ng/L of chlorinated organic pesticides in water samples and 364 ng/g in sediment samples were estimated. Whereas, the concentration of these compounds in fish samples is as high as 137 ng/g. Key words: Chlorinated Organic Pesticide Monitoring; Water, Sediments and Fish INTRODUCTION Aquatic pollution by toxic chemicals is a global problem. Various activities such as farming, fishing, forestry, construction, mining, urban development and land pollution occurring in or near the watershed of a reservoir could bring about water quality problems and disruption in fish (Moshood, 2009). Chlorinated Organic Pesticides are very stable in both fresh and salt water and is resistant to photo degradation (Shokrzadeh and Zehtab, 2009). They will disappear from the water with secondary mechanisms such as, absorption on sediment, biological breakdown by microflora and fauna, and absorption by fish through gills, skin and feeding. They are poorly hydrolyzed and slowly biodegrades in environment. Therefore, these compounds are persistent in food chains and are readily accumulated in animal tissues. Fish absorb these compounds directly by water or by ingesting contaminated food. In particular, Organochlorine insecticides and PCBs are highly stable under different environmental conditions and persistent nature and chronic adverse effects on wildlife and humans (Monirith, et al, 2000). Diabetes, also, has been associated with dioxin-like chemicals,non-dioxin-like PCBs,DDE, and/or other organochlorine pesticides in several cross-sectional investigations sectional investigations species composition (Mary Turyk, et al., 2009). Different studies proved that residues of organic chlorinated pesticides do exist in some water resources in Egypt, and particularly in Manzala Lake (Abou-Arab, et al., 1995). In the same time none is considered concerning residues in fish farms that use water drainage of Bahr El-Bakar (Hassan, et al.1996). The water from this drainage consists of a mixture of domestic, agricultural, and industrial wastewater (El-Mekkawi, et al., 1996). Therefore, the current study aims to discover the amount of water contamination in these fish farms that are located at Sahl Al-Husainia, Sharkia governorate, and its concentrations in fish tissues breaded in these waters. MATERIALS AND METHODS Studied Organic Chlorinated Pesticides: Alpha, B, H, C, Lindane, Alderine, D-Alderine, DDT and its derivatives (DDI, DDD, Ortho Para DDT) pesticide residues were studied. Solutions of these pesticides were prepared by dissolving definite weights of pure active ingredients (obtained from Ultra Scientific 250, Smith Street, North Kingdom, RIO2852) in hexane. Chemicals: High grade pure organic solvents were used to carry out this study obtained from BDH Company,