International Journal of Livestock Research eISSN : 2277-1964 NAAS Score -5.36 Vol 7 (5) May’17 Hosted@www.ijlr.org DOI 10.5455/ijlr.20170330053039 Page209 Original Research Evaluation of Anthelmintic Activity (In -vitro) of Boerhaavia diffusa Linn (Punarnava) Root Extract Including Phytochemical Screening Shelu R. S., Jangde, C. R., Nanotkar, R. Y. 1* , Limsay, R. P., Awasthy, V. B. and Singh, R. P. 2 Department of Veterinary Pharmacology and Toxicology, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University (MAFSU), Nagpur-07, Maharashtra, INDIA 1 Department of Veterinary Pharmacology and Toxicology, West Bengal University of Animal and Fishery Sciences, K.B.Sarani-37, Kolkata, West Bengal, INDIA 2 Department of Veterinary Pharmacology and Toxicology, Nanaji Deshmukh Veterinary Science University, Jabalpur (Madhya Pradesh), INDIA *Corresponding author: drrahul923vet@gmail.com Rec. Date: Feb 14, 2017 18:27 Accept Date: Mar 30, 2017 17:30 Published Online: May 01, 2017 DOI 10.5455/ijlr.20170330053039 Abstract The plant Boerhaavia diffusa is a herb found throughout the India. The roots of the plant have been described in various herbal natural medicine literatures to possess many medicinal properties like anti- inflammatory, antibacterial, diuretic, antiurethritis, hepatoprotective, anti-convulscent, antinematodal and antifibrinolytic etc. In view of various medicinal uses, the root of the plant was selected for screening of anthelmintic property. The helminths including Monezia expansa, Haemonchous contortus and Amphistomes were used for study. The B. diffusa alcoholic extract at 10, 20, 30 mg/ml concentration can produced paralysis and death of worm within half to five hrs of exposure. Plant root extract at higher dose (30mg/ml) reported significant anthelmintic activity by causing paralysis and death, when compared to standard drug against three of heminths. The extractability percentage with absolute alcohol was worked out to be 8%. The phytochemical screening reveals the presence of sterol, alkaloid, amino acid, protein, reducing sugar, glycoside, saponins, tannins, and flavonoides. This study provides scientific evidence that the root of B diffusa has anthelmintic property. Key words: Anthelmitic, Boerhaavia diffusa Linn, Helminths, Paralysis How to cite: Shelu, S., Jangde, C., Nanotkar, R., Limsay, R., Awasthy, V., & Singh, R. (2017). Evaluation of Anthelmintic Activity (In -vitro) of Boerhaavia diffusa Linn (Punarnava) Root Extract Including Phytochemical Screening. International Journal Of Livestock Research, 7(5), 209-219. http://dx.doi.org/10.5455/ijlr.20170330053039