1760 Indian Journal of Forensic Medicine & Toxicology, January-March 2021, Vol. 15, No. 1 Comparison of Success Rate of Estrus and Pregnancy between Laser Puncture and Intra Vaginal Progesterone Sponge in Bali Cattle R. Tatang Santanu Adikara 1 , Herry Agoes Hermadi 2 , Warsito Sunaryo Hadi 3 , Lita Rakhma Yustinasari 4 1 Professor, Department of Veterinary Anatomy, 2 Professor, Department of Reproduction, 3 Lecturer, Department of Animal Nutrition, 4 Lecturer, Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia. Abstract The purpose of this study was to fnd a method of determining laser models and intra vaginal progesterone sponge in Bali Cattle. An assessment was carried out at Sapi Loka Grati, Pasuruan, Indonesia. The parameters observed included the symptoms of estrus, response of estrus, and the level of pregnancy. Twenty Balinese cows were divided at random in two groups. P1 treated with laser puncture at a certain point and P2 treated with 1.5 g of progesterone + 10 mg of estradiol benzoate intra vaginal sponge. The diagnosis of subsequent pregnancies was done by ultrasonographic examination on day 40 th after artifcial insemination (AI). The laser used in this study was a soft laser with power supply specifcations as follows: 50 Hz, 220 Volts, 50 Watts, ranging from 0.2 Joules to 0.5 Joules. These lasers include semiconductor lasers and laser probes. Three acupuncture points were used (GV 4, BL-22, and GV-2). Furthermore, the intra vaginal progesterone sponge used was made from silicone plastic similar to the drugs containing medroxyprogesterone acetate. Data testing was done by using the T-test. Results demonstrated that the P1 responses of estrus appeared on day 3 rd , while P2 on day 9 th . There was no signifcant difference (p >0.05) in the accumulation of estrus and pregnancy. In conclusion, estrus synchronization and pregnancy rate could be effciently achieved in Bali cattle using either laser puncture or intra vaginal progesterone sponge. However, laser puncture treatment induced more spread estrus with higher pregnancy rate. Keywords: Bali cattle, estrus synchronization, intra vaginal progesterone, laser puncture, pregnancy. Introduction Bali cattle is one of the beef-producing livestock in Indonesia, but domestic Balinese beef production has not been able to cover the needs because of their population and low productivity levels. Various methods have been taken so that livestock productivity is maximized, including through improved management and quality of feed or by utilizing technology. The low population of Bali cattle is partly due the low level of reproduction 1 . For better reproductivity, estrus induction is one of important ways to be performed. Estrus induction is usually performed with hormonal method, such as application of PGF2α, but the disadvantage is relatively expensive 2 . Laser puncture technology has been proven to induce estrus with variable success in goats 2,3 , sheep 4 , and cows 5 . Irradiation of acupoints by means of low- level laser provides a relatively safe, noninvasive and effcient way of stimulation of acupoints 6 . Response and signs of estrus could apparently be improved after conducting a laser puncture procedure, so that could increase a pregnancy rate 7,8 . The application of laser puncture to Bali cows resulted in a response to estrus 90%, and from insemination, a pregnancy rate of 80% was obtained. The occurrence of conception in marriage indicates that estrus that occurs with laser puncture stimulation is followed by ovulation 3 . In this study, laser Corresponding author: Herry Agoes Hermadi Email: herrypro59@yahoo.com