J. Bio. & Env. Sci. 2013 17 | Sabariswaran et al. RESEARCH PAPER OPEN ACCESS Bio-hydrogen Production from sago effluent Kandasamy Sabariswaran 1* , Paramasamy Papitha 2 , Ravichandran Indumathi 2 , Sundararaj Selvakumar 1 , Samuel Paul Raj 2 1 Department of Environmental Studies, School of Energy, Environment and Natural Resources, Madurai Kamaraj University, Madurai – 625 021, Tamil Nadu, India 2 Department of Natural Resources and Waste Recycling, School of Energy, Environment and Natural Resources, Madurai Kamaraj University, Madurai – 625 021, Tamil Nadu, India Article published on February 18, 2013 Key words: Bio-hydrogen, sago effluent, cow dung inoculums, substrate. Abstract The present study focuses on the exploitation of Sago effluent as a source for hydrogen production. Hydrogen production was investigated at different parameters namely pH, Temperature and substrate. The pH was varied from 4.0 - 6.5, temperature ranges from 30°C - 70°C and substrate concentration (Glucose and Nitrogen) varied from 0.25 g/l - 1.25 g/l and their interaction on hydrogen gas production were studied. The raw cow dung was used as inoculums for hydrogen gas production. In this study revealed that the maximum hydrogen production was occurred in acidic condition (pH 5.5). The highest rate of hydrogen production was observed at 1.25 g/l of substrate concentration (both Glucose and Nitrogen) under 55 °C. * Corresponding Author: Kandasamy Sabariswaran  sabariswaran14@gmail.com Journal of Biodiversity and Environmental Sciences (JBES) ISSN: 2220-6663 (Print) 2222-3045 (Online) Vol. 3, No. 2, p. 17-23, 2013 http://www.innspub.net