~ 31 ~ The Pharma Innovation Journal 2020; SP-9(12): 31-35 ISSN (E): 2277- 7695 ISSN (P): 2349-8242 NAAS Rating: 5.03 TPI 2020; SP-9(12): 31-35 © 2020 TPI www.thepharmajournal.com Received: 21-10-2020 Accepted: 24-11-2020 Rajneesh M.V.Sc. Scholar, Livestock Production Management Section, NDRI, Karnal, Haryana, India AK Misra Principal Scientist, Livestock Production Management Section, NDRI, Karnal, Haryana, India Shwetambri Jamwal M.V.Sc. Scholar, Livestock Production Management Section, NDRI, Karnal, Haryana, India Prince Chauhan Ph.D. Scholar, Animal Nutrition department NDRI, Karnal, Haryana, India Ninad Bhatt Ph.D. Scholar, Livestock Production Management Section, NDRI, Karnal, Haryana, India Corresponding Author: Rajneesh M.V.Sc. Scholar, Livestock Production Management Section, NDRI, Karnal, Haryana, India Blood profile of early lactating Murrah buffaloes supplemented with bypass fatty acids and Tinospora cordifolia Rajneesh, AK Misra, Shwetambri Jamwal, Prince Chauhan and Ninad Bhatt Abstract The study was conducted with the objective of evaluating the effect of bypass fatty acid and Tinospora cordifolia supplementation on blood profile of early lactating Murrah buffaloes. The lactating buffaloes were distributed into four groups, each having five buffaloes depending on previous milk yield, parity and body weight. T1, T2 and T3 group buffaloes were supplemented for 90 days with 150g bypass fatty acids, 150g Tinospora stem powder and a combination of both 150g bypass fatty acids and 150g Tinospora stem powder respectively while the control group (T0) given only standard feeding without any supplementations. Blood sampling was done at fortnightly intervals and results showed that among hematological parameters TLC, neutrophil count, N:L and platelet counts were significantly higher in Tinospora supplemented groups (T2 and T3) whereas values of RBC, Hb, PCV, lymphocytes and mix cells were not affected significantly. The blood indices were also not changed significantly on supplementation. From results, it was concluded that change in blood profiling was there due to the immunomodulatory effect if Tinospora cordifolia. Keywords: Buffaloes, blood, bypass fatty acid, supplementation, tinospora Introduction The success of dairy farming depends on the production and health of animal during lactation. Transition period is most crucial from production and health point of view. It begins three weeks before parturition and remains up to three weeks after parturition. During this period, severe negative energy balance and low serum or plasma concentrations of several minerals and vitamins are experienced by animal which is an indicative of lowered health status [1] . During the early stage of lactation, the health and immune system of animals gets compromised [2] . The main reason for this is negative energy balance (NEB), which is due to the difference between the energy requirements and availability [3] . The maintenance and production requirements of high producing animals are not fulfilled with the regular dietary sources; therefore, extra energy supplementation is required to fulfil the maintenance and production demand of the lactating animals. Supplementation of bypass fat ensures the availability of high energy [3-6] , whereas, Tinospora cordifolia can serve as immunity booster for animals [7, 8] . Blood profiles of animals are used to evaluate the health status and ongoing changes inside the animal’s body [9] . Analysis of specific blood metabolites and their values allows the determination of accuracy of different metabolic pathways associated with energy, protein, and minerals and showed the true picture of animal health and this can be utilized to optimize the production and health of dairy animals [10, 11] . Considering this the study was conducted to analyze the blood profiles of lactating Murrah buffaloes on supplementation with bypass fatty acid and Tinospora cordifolia. Material and methods Description of the study area The following experiment was conducted in the Livestock Research Centre of National Dairy Research Institute (N.D.R.I.), Karnal, Haryana, India which is located 29°42” North and 79°54” East longitudes at an altitude of 245 meters above the mean sea level in the beds of Indo-Gangetic alluvial plain. The area has a minimum temperature that falls near to freezing point in winter and goes maximum to 45 o C in months of May/June in summer.