Colocalization of Cancer Associated Biomarkers on Single Extracellular Vesicles for Early Cancer Detection, Salem DP, Bortolin LT, et. al. 1 Colocalization of Cancer Associated Biomarkers on Single Extracellular Vesicles for Early Cancer Detection Daniel P. Salem 1 * # , Laura T. Bortolin 1 *, Anthony D. Couvillon 1 , Dan Gusenleitner 1 , Jonian Grosha 1 , Ibukunoluwapo O. Zabroski 1 , Kelly M. Biette 1 , Sanchari Banerjee 1 , Christopher R. Sedlak 1 , Delaney M. Byrne 1 , Bilal F. Hamzeh 1 , MacKenzie S. King 1 , Lauren T. Cuoco 1 , Peter A. Duff 1 , Emily S. Winn-Deen 1 , Eric K. Huang 1 , Randy Schekman 2 , and Joseph C. Sedlak 1# #Co-corresponding Authors *Co-first authors Affiliations: 1 Mercy BioAnalytics Inc. 22 Strathmore Road, Natick, Massachusetts 01760 (USA) 2 HHMI Investigator, Dept. of Mol. and Cell Biology, Li Ka Shing Center, UC Berkeley, Berkeley, CA, 94720-3370 (USA) Acknowledgements: David Ransohoff, Steven Skates, Floyd Taub, Dawn Mattoon, Daria Filonov, Ed Ha The results published here are in whole or part based upon data generated by the TCGA Research Network: https://www.cancer.gov/tcga. The Genotype-Tissue Expression (GTEx) Project was supported by the Common Fund of the Office of the Director of the National Institutes of Health, and by NCI, NHGRI, NHLBI, NIDA, NIMH, and NINDS. The RNA sequencing data (GTEx Analysis V8) used for the analyses described in this manuscript were obtained from the GTEx Portal on 04/08/2021. . CC-BY-NC-ND 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprint this version posted February 8, 2023. ; https://doi.org/10.1101/2023.02.07.527360 doi: bioRxiv preprint