45 ISSN 2808-3229 Journal of Biodiversity and Biotechnology. 2(2), 45–52, 2022 URL: https://jurnal.uns.ac.id/jbb/article/view/63262 doi: http://dx.doi.org/10.20961/jbb.v2i2.63262 Application of Livestock Waste Liquid Fertilizer and Mychorriza Biofertilizer on The Growth of Biduri (Calotropis gigantea) Samanhudi 1,2* , Amalia Tetrani Sakya 2 , Sri Hartati 1,2 , Muji Rahayu 2 , Anne Sukmawati 4 , and Muhammad Naufal Adani 3 1 Center for Biotechnology and Biodiversity Research and Development, Institute of Research and Community Services, Universitas Sebelas Maret, Jl. Ir. Sutami 36A Kentingan Jebres Surakarta 57126, Indonesia 2 Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret. Jl. Ir. Sutami 36A, Surakarta 57126, Central Java, Indonesia. 3 Study Program of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret, Jl. Ir. Sutami 36A Surakarta 57126 - Indonesia 4 Center for Textile, Agency for Standardization and Industrial Services Policy, Jl. Jend. A. Yani 390 Bandung 40272 – Indonesia * Corresponding author: samanhudi@staff.uns.ac.id Abstarct Biduri (Calotropis gigantea) was a plant with several benefits, namely as a medicinal plant and textile material. Biduri was a wild plant that could grow in various soil conditions. The existence of biduri still needed to be fully utilized by many people. So it was necessary to develop the cultivation technology of Biduri to take advantage of this plant. This research was conducted at the field laboratory of Jumantono, Faculty of Agriculture, Universitas Sebelas Maret, Karanganyar, and used a factorial Randomized Completely Block Design (RCBD), which consisted of 2 factors, namely organic liquid fertilizer from livestock waste and mycorrhizae. Livestock waste liquid fertilizer consists of 40ml/kg soil cow urine, 40ml/kg soil goat urine, 40ml/kg soil rabbit urine, and 40ml/kg soil fish waste. Mycorrhizae consisted of 0 grams/plant, 5 grams/plant, 10 grams/plant, and 15 grams/plant. There were 20 treatment combinations repeated three times, so the total was 60 treatment combinations. The interaction between livestock waste liquid fertilizer and mycorrhizae did not significantly affect all the physiological observation variables. The results showed that the application of livestock waste liquid fertilizer had no significant effect on all observations of physiological variables. Giving mycorrhizae shows that it can increase the number of stomata and the width of the stomata aperture. Keyword: Biduri; urine of cattle; fish waste; mycorrhizae; physiological Cite this as: Samanhudi, Sakya, A.T., Hartati, S., Rahayu, M., Sukmawati, A., Adani, M.N. (2022). Application of Livestock Waste Liquid Fertilizer and Mychorriza Biofertilizer on The Growth of Biduri (Calotropis gigantea). Journal of Biodiversity and Biotechnology. 2(2), 45–52. doi: http://dx.doi.org/10.20961/jbb.v2i2.63262 Introduction Biduri (Calotropis gigantea) is a herbaceous plant that can grow wild and reproduce quickly. (1) stated that this plant can grow freely in various places such as calcareous soil, hard soil, highlands, and beaches, so the existence of biduri can be found in various places. Biduri is also a medicinal plant that has many benefits. Leaves, stems, flowers, sap, and roots can be utilized. According to (2), biduri has benefits as herbal medicine that can relieve coughs, toothaches, diarrhea, and itching. The biduri plant has been used by most people and has begun to be sought for some