Vol 15, Issue 10, 2022 Online - 2455-3891 Print - 0974-2441 PHYTOCHEMICAL SCREENING WITH LC-HRMS PROFILING AND IN VITRO BIOLOGICAL ACTIVITIES OF ARGYREIA CUNEATA (L.) AND ARGYREIA SETOSA (L.). SANDIP KALE 1 , PRANOTI KIRDAT 1 , SURESH KALE 2 , PADMA DANDGE 1 * Received: 13 June 2022, Revised and Accepted: 25 July 2022 ABSTRACT Objective: The present study was designed for phytochemical screening and biological activities of Argyreia cuneata (L.) and Argyreia setosa (L.) medicinal plants. Methods: The mature leaves of A. cuneata (L.) and A. setosa (L.) were extracted with methanol (Ac-Me, As-Me) and ethyl acetate (Ac-EA, As-EA) solvent followed by qualitative and quantitative phytochemical analysis. Antimicrobial activity was evaluated by agar well diffusion method, whereas the activities such as antioxidant, antidiabetic, and anti-inflammatory were determined by in vitro methods. Liquid chromatography-high-resolution mass spectroscopy (LC-HRMS) was used to recognize the bioactive components. Results: The highest phenolic content (0.840±0.130 mg GAE/ml extract) reported from As-Me and for flavonoids 0.128±0.012 mg QE/ml from Ac- EA. The Ac-Me exhibited higher inhibition zone against all the bacteria used for study. In antioxidant activity, Ac-Me and Ac-EA report highest 2,2 dipheny l,1 picryl hydrazine (IC50=0.580±0.012 mg/ml) and nitric oxide radical scavenging potential (IC50=0.772±0.059 mg/ml), respectively. For antidiabetic activity, As-Me showed highest α-amylase inhibition activity as well as glucose adsorption. In anti-inflammatory activity, Ac-EA exhibits highest (IC50=0.529±0.009 mg/ml) protein denaturation inhibition and Ac-Me showed highest (91.56±1.96%) HRBC hemolysis inhibition. The LC- HRMS analysis of methanolic extract reports the majority of phenolic compounds. Conclusion: The study showed that the plants A. cuneata (L.) and A. setosa (L.) are well exploited and can be used for the source of potent natural bioactive components. This study also may thereby provide an insight in screening of crude drug. Keywords: Argyreia cuneata, Argyreia setosa, Liquid Chromatography–High-Resolution Mass Spectroscopy, Biological activities, DPPH. INTRODUCTION Phytochemical analysis has great interest to identity the health benefits in drug and functional food development. The health effects of phytochemicals can identify by many in vitro screening methods. Due to high cost and low productivity in these methods, there is scope for improvement. Hence by utilizing molecular, chemical, or pharmacological information, researchers have proposed in silico methods as alternative [1,2]. The medicinal plants are the great source of essential nutrients and bioactive phytochemicals which are important in promoting health as well as preventing diseases. The vital nutrients, that is, macro- and micronutrients in plants address as crucial requirement for human health since long ago. Recently, the phytochemicals emerged as key molecules of various cellular signaling pathways that known to be secondary metabolites. These compounds are generally non-nutritive chemicals produced through several chemical pathways in plants. The large number of phytochemicals can be beneficial to the cellular functions. It also shows the effects on heath as phytochemical rich food and strongly indicates their role in curing diseases. The plant genus Argyreia (L) belongs to family convolvulaceae that has been used in different system of traditional medication for the treatment of diseases and ailments of human beings. The ergot alkaloid lysergic acid amide (LSA) is a secondary plant constituent in a number of plants, but it is mainly present in considerable amounts in Convolvulaceae, like Argyreia nervosa. The LSA level in correlation with several vegetative adverse effects in human serum and urine analyzed [3]. The anti-diabetic potential and antibacterial activity of silver nanoparticles synthesized from A. nervosa have been evaluated. The preliminary phytochemical analysis of several species from genus Argyreia is done [4,5]. Argyreia speciosa possessed strong antioxidant activity, remarkable anti- diabetogenic effect comparable to glibenclamide, a well-known liver protecting herbal. The methanolic extract of Argyreia argentea shows potent analgesic and anti-inflammatory activity in animal model. The extract could be a new and potential source of anti-inflammatory and analgesic drug [6,7]. Three resin glycosides (two are isomers) are isolated from Argyreia acuta. N-Formylloline is isolated from roots of Argyreia mollis and this is the first identification of a 1-aminopyrrolizidine alkaloid (loline alkaloid) in a species of the Convolvulaceae [8,9]. A. nervosa contains the presence of some major phytochemicals such as alkaloids, glycosides, tannins, and flavonoids. The Butanolic extract of A. speciosa showed potent antiulcer property in vitro and in vivo study. The antiulcer potential of n-butanol fraction of methanolic extract of A. nervosa may be due to the synergistic effect of Quercetin and Kaempferol [10-12]. The ethanolic extract of A. speciosa possesses immunomodulatory activity. The compounds such as hexadecanyl p-hydroxycinnamate and scopoletin isolated from A. speciosa roots showed the antifungal activity. A. speciosa possesses significant dose- dependent gastroprotective activity, probably due to its free radical scavenging activity [13-15]. The literature survey revealed that the chemical diversity of bioactive components and pharmacological actions of many species for genus Argyreia remains unaddressed by rigorous scientific research to define efficacy and safety. Hence, our study focusing on to reports the active phytochemicals from the A. cuneata (L.) and A. setosa (L.) plants which show the biological activities. METHODS Collection of sample and preparation of extract The A. cuneata (L.) and A. setosa (L.) plant samples were collected from western hat region covered under Junnar Tahsil of Pune © 2022 The Authors. Published by Innovare Academic Sciences Pvt Ltd. This is an open access article under the CC BY license (http://creativecommons.org/ licenses/by/4.0/) DOI: http://dx.doi.org/10.22159/ajpcr.2022v15i10.45502. Journal homepage: https://innovareacademics.in/journals/index.php/ajpcr Research Article 1 Department of Biochemistry, Shivaji University, Kolhapur, Maharashtra, India. 2 Department of Botany, Sathaye College, Vile Parle, Mumbai, Maharashtra, India. E-mail: ssk.biochem@unishivaji.ac.in