Research Article Factors Associated with Utilization of HIV Testing Services among Adolescents Aged 10-19 Years in Lira District, Northern Uganda: A Cross-Sectional Study Deo Benyumiza, Joan Fidelia Amongin, Isaac Ochaba, Morish Adupa, Naume Abuch, Constance Babirye Banula, and Samson Udho Faculty of Health Sciences, Lira University, P.O. Box 1035, Lira, Uganda Correspondence should be addressed to Samson Udho; sudho@lirauni.ac.ug Received 9 May 2021; Revised 15 July 2021; Accepted 3 August 2021; Published 12 August 2021 Academic Editor: Mateusz Maciejczyk Copyright © 2021 Deo Benyumiza et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Background. HIV testing remains a problem among adolescents in low- and middle-income countries, and yet, HIV testing is a cornerstone in the fight against HIV. However, there is scanty literature on the utilization of HIV testing services by adolescents especially in rural settings. This study is aimed at determining the uptake of HIV testing services and associated factors among adolescents aged 10-19 years in Lira District, Northern Uganda. Methods. This was a cross-sectional study done among 277 randomly selected adolescents aged 10-19 years attending outpatient clinics in Pentecostal Assembly of God (PAG) Mission Hospital, Ngetta Health Center III, and Boroboro Health Center III. Data were collected using an interviewer-administered structured questionnaire. Data collected included sociodemographic characteristics, history of test and receipt of HIV results in the last three months, and facility-related factors affecting uptake of HIV testing services. Data analysis consisted of descriptive statistics, cross-tabulations, and logistic regression at a 95% level of significance in SPSS version 25. Results. The uptake of HIV testing services was 43% (119/277) among the study participants. Adolescents who had completed primary education (aOR: 5.47; 95% CI: 1.07-28.15; p =0:042), are employed (aOR: 2.77; 95% CI: 1.16-6.60; p =0:022), had used a condom in the last sexual intercourse (aOR: 4.46; 95% CI: 1.78-11.15; p =0:001), and are involved in HIV testing outreaches (cOR: 10.86; 95% CI: 3.81-30.93; p ≤ 0:001) were more likely to uptake HIV testing services compared to those who had tertiary education, are unemployed, had never used a condom, and are not involved in HIV testing outreaches. Conclusion. Utilization of HIV testing services by adolescents aged 10-19 in Lira District, Northern Uganda, is generally low. The Ministry of Health should strengthen HIV testing services targeting adolescents to increase uptake of HIV testing services. 1. Introduction HIV/AIDS remains a pandemic of public health interest [1]. Globally, it is estimated that 1.7 million adolescents between ages 10 and 19 are living with HIV with 170,000 new infec- tions and 34,000 AIDS-related deaths [2]. Sub-Saharan Africa accounts for 73% of new HIV infections among ado- lescents occurring globally [2]. In Uganda, the prevalence of HIV among adolescents is still high at 3.3%, and yet, adoles- cents are key to achieving HIV targets in Uganda [3]. HIV continues to disproportionately affect the adolescent at a global, regional, and national level [2]. Adolescents are more vulnerable to HIV infection because of their risky sex behav- iors, inexperience, and invincible tendency [4, 5]. HIV testing is a window for all HIV-related care and treatment services and an essential step in achieving “the UNAIDS 90-90-90 targets” among all age groups [5]. How- ever, globally, only 35% of young people were aware of their HIV status in 2015 [6]. In sub-Saharan Africa, only 13% of female and 9% of male adolescents tested for HIV and received their results in the last 12 months [7]. In Uganda, only 39.4% of older adolescents (15-19 years) had tested and received their HIV results in the past 12 months [8]. This has prompted the government of Uganda through the Hindawi BioMed Research International Volume 2021, Article ID 9568148, 7 pages https://doi.org/10.1155/2021/9568148