Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Thu, 01 Nov 2018 04:56:24 Sphingomicrobium lutaoense gen. nov., sp. nov., isolated from a coastal hot spring Peter Ka ¨ mpfer, 1 A. B. Arun, 2 Chiu-Chung Young, 3 Hans-Ju ¨ rgen Busse, 4 Johannes Kassmannhuber, 4 Ramon Rossello ´ -Mo ´ ra, 5 Birgit Geueke, 6 P. D. Rekha 2 and Wen-Ming Chen 7 Correspondence Peter Ka ¨ mpfer peter.kaempfer@umwelt.uni- giessen.de 1 Institut fu ¨ r Angewandte Mikrobiologie, Justus-Liebig-Universita ¨ t Giessen, D-35392 Giessen, Germany 2 Yenepoya Research Center, Yenepoya University, University Road, Deralakatee, Mangalore, Karnataka State, India 3 Department of Soil & Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan, ROC 4 Institut fu ¨ r Bakteriologie, Mykologie und Hygiene, Veterina ¨ rmedizinische Universita ¨ t, A-1210 Wien, Austria 5 Marine Microbiology Group, Institut Mediterrani d’Estudis Avanc ¸ ats (CSIC-UIB), C/Miquel Marque ´s 21, 07190 Esporles, Spain 6 Environmental Microbiology, Eawag, Ueberlandstrasse 133, CH-8600 Du ¨ bendorf, Switzerland 7 Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung, Taiwan, ROC A yellowish pigmented, Gram-negative, rod-shaped, non-spore-forming bacterium (strain CC- TBT-3 T ), was isolated on marine agar 2216 from a coastal hot spring of Green Island (Lutao), located off Taituang, Taiwan. 16S rRNA gene sequence analysis of strain CC-TBT-3 T showed a relatively low similarity (,95.5 %) to representatives of the genera Novosphingobium, Sphingosinicella and Sphingomonas of the Sphingomonadaceae, with the most related strain being the type strain of Novosphingobium soli. In addition to the relatively low 16S rRNA gene sequence similarity to members of established species, the isolate also showed some unique chemotaxonomic features, including the presence of some glycolipids with unusual chromatographic behaviour. The major components of the polar lipid profile were diphos- phatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid and three unidentified glycolipids. The major respiratory quinone was ubiquinone Q-10. The polyamine pattern was characterized by the triamine sym-homospermidine as a major component. Although the predominant fatty acids were C 18 : 1 v7c and summed feature 3 (C 16 : 1 v7c and/ or iso-C 15 : 0 2-OH), the isolate did not show the typical hydroxyl fatty acids, such as C 14 : 0 2- OH, C 15 : 0 2-OH and C 16 : 0 2-OH, found in members of the genera Novosphingobium, Sphingomonas and Sphingosinicella, but showed instead high amounts of C 18 : 1 2-OH (12.0 %). The DNA G+C content of strain CC-TBT-3 T was 63.4 mol%. 16S rRNA gene sequence, chemotaxonomic and physiological analyses revealed that strain CC-TBT-3 T represents a novel species in a new genus in the family Sphingomonadaceae for which the name Sphingomicrobium lutaoense gen. nov., sp. nov. is proposed; the type strain is of the type species S. lutoaense, CC-TBT-3 T (5DSM 24194 T 5CCM 7794 T ). On the basis of 16S rRNA gene sequence and chemotaxo- nomic analyses, Takeuchi et al. (2001) proposed a dissec- tion of the former genus Sphingomonas (Yabuuchi et al., 1990, 1999), and established the genera Sphingobium, Novosphingobium and Sphingopyxis. Maruyama et al. (2006) added an additional genus, Sphingosinicella, to this group of Abbreviations: pNA, para-nitroanilide; pNP, para-nitrophenyl. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CC-TBT-3 T is EU564841. Three supplementary figures are available with the online version of this paper. International Journal of Systematic and Evolutionary Microbiology (2012), 62, 1326–1330 DOI 10.1099/ijs.0.034413-0 1326 034413 G 2012 IUMS Printed in Great Britain