~ 760 ~ Journal of Pharmacognosy and Phytochemistry 2017; SP1: 760-763 E-ISSN: 2278-4136 P-ISSN: 2349-8234 JPP 2017; SP1: 760-763 Rohit Lall Department of Molecular and Cellular Engineering, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad U.P, India Poonam singh Department of Molecular and Cellular Engineering, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad U.P, India Kirti Singh Department of Molecular and Cellular Engineering, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad U.P, India Anupam Jha Department of Molecular and Cellular Engineering, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad U.P, India Vartika Gupta Department of Molecular and Cellular Engineering, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad U.P, India Shraddha Nagar Department of Molecular and Cellular Engineering, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad U.P, India Correspondence Rohit Lall Department of Molecular and Cellular Engineering, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad U.P, India Comparative study of nutritional, antimicrobial and antioxidant properties of Pleurotus ostreatus and Agaricus bisporus Rohit Lall, Poonam singh, Kirti Singh, Anupam Jha, Vartika Gupta and Shraddha Nagar Abstract The phytochemical analysis of two very common commercially grown varieties of mushrooms i.e.,: Pleurotus ostreatus & Agaricus bisporus was donein the present study. Antimicrobial property of mushroom extract was studied for various gram positive and gram negative bacteria ( E. coli, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus). The extracts show antimicrobial property against all tested bacterial strains and it reveals that mushroom was rich with antioxidants. The presence of alkaloids, carbohydrates, flavanoids, amino acids, vitamins (ascorbic acid) and phenolic compounds has been identified quantitatively these compounds are estmitated by spectrophotometer. Phytochemical investigation indicated the presence of high concentration of carbohydrates, amino acids, vitamin c, phenols, flavanoids, alkaloids in case of oyster mushroom while high concentration of protein and tannin was found in button mushroom. The reducing power assay indicated that Agaricus bisporush has high antioxidant potency with 0.3mg/ml concentration with highest absorbance. This study supports that mushroom can be used as a very nutritive source for various primary and secondary metabolites and can also be used as treatment of antibacterial disease. Keywords: Pleurotus ostreatus, Agaricus bisporus, spectrophotometer, anti microbial activity, anti oxidants Introduction The two most extensively used varieties of mushroom are Agaricus bisporus (Button mushroom) and Pleurotus ostreatus (Oyster Mushroom). Are a wide variety of phytochemicals (Parihar et al., 2016) [16] . The Button Mushroom, is the most important commercially cultivated mushroom all over the world. Research findings demonstrate that the button mushroom possesses bioactive components of nutritional importance (Rezaeian and Pourianfar 2015) [21] . Agaricus bisporous (white button mushroom) is a reported to have antimicrobial property against various pathogenic bacteria which causes lethal diseases whereas oyster mushroom comprises of various primary and secondary metabolites (alkaloids, tannins, flavanoids, phenols) in a very good amount (Han et al., 2015) [8] . Mushroom also comprises of various anti microbial, anti fungal, anti viral, anti tumour and anti oxidant compounds effective against potent diseases (Dharmaraj et al., 2014) [6] . AST is used to determine sensitivity of antibiotic compounds against various pathogens (Iwalokun et al., 2007) [10] . Since synthetic antioxidants are sometimes toxic in nature, therefore a necessity of strong natural antioxidant (Muthangya et al., 2014) [14] . In the current study will examine the nutritional value of the two commercially used mushroom species namely, Agaricus bisporus and Pleurotus ostreatus. The primary metabolites and secondary metabolites are to be analyzed quantitatively and quantitatively. Further the antimicrobial properties of mushroom will be verified. Materials and Methods Collection of Mushroom Fresh mushroom varieties Pleurotus ostreatus (oyster mushroom) were collected from organic farm of SHIATS, Allahabad, India and collection of Agaricus bisporus (white button mushroom) from market of Allahabad. Preparation of Mushrooms Powder Mushrooms were initially cleaned, then sliced into small pieces. They were dried in hot air oven at 45 °C for 2 hr and grinded in mixer-grinder to make fine powder. They were stored.