ISPUB.COM The Internet Journal of Forensic Science Volume 3 Number 2 1 of 4 Study For The Development Of Isolation And Clean Up Process By Using Solid Phase Extraction (SPE) For Fluoroquinolones And Cephalosporins Antibiotic Drugs From Forensic Samples V Mishra, J Sharma, S Shukla Citation V Mishra, J Sharma, S Shukla. Study For The Development Of Isolation And Clean Up Process By Using Solid Phase Extraction (SPE) For Fluoroquinolones And Cephalosporins Antibiotic Drugs From Forensic Samples. The Internet Journal of Forensic Science. 2008 Volume 3 Number 2. Abstract A new and sensitive sample clean up method is been developed for the extraction of the antibiotic drugs of cephalosporin and fluoroquinolone group from forensic toxicological samples (Blood, Urine and tissues). The method is simple and easy to do in normal routine analysis in the laboratory. . The suggested solid phase extraction (SPE) method is simple and time efficient for easy and fast cleaning of biological samples for quality sample preparation. The C-18 cartridges were used in the process and the average recovery of these cephalosporin and fluoroquinolone antibiotic drugs after sample clean up was >90% for in urine and > 80% in blood and tissue samples for forensic analysis. INTRODUCTION The principle aim of toxicological methods of ‘Isolation and Clean Up’ is to provide an ideal concentrate to which the chromatographic and other instrumental methods of identification as well as chemical reactions can be applied. Before applying methods of detection and determination in forensic samples, it is necessary to apply appropriate sample clean up procedure which should be simple, economic and result oriented during the examination of biological samples. The various biological samples including mainly Blood, urine, stomach wash, spleen, liver and kidney tissues contains coagulated proteins and large amount of fats which interferes with the chemical activity of target drug present in the matrix, moreover this interference induces improper or no identification of the drugs in the matrix during qualitative and quantitative analysis of any drug in forensic samples. Various authors have used the solid phase extraction procedures [ 123456 ] for the extraction of drugs from different types of samples. Under the following study, a sample clean up method is being proposed for the separation of fluoroquinolones and cephalosporin antibiotics in forensic samples. The suggested solid phase extraction (SPE) method is simple and time efficient for easy and fast cleaning of biological samples for quality sample preparation MATERIAL AND INSTRUMENTATION The different biological samples including blood, urine and tissues (Stomach, spleen, kidney and liver) were spiked with known quantity of standard antibiotic drug samples and placed in incubator for overnight to let the interaction of antibiotic sample and the biological matrix. The C18 SPE cartridge, a Solid Phase Extractor (SPE) instrument (Manual operation), acetonitrile, methanol, 2M-perchloric acid, Whatman filter paper No.42 HPLC grade water, a centrifuge and a Nitrogen evaporator is used for clean up and sample preparation process. PROCEDURE OF SAMPLE CLEAN UP AND PREPARATION FOR CEPHALOSPORIN ANTIBIOTIC DRUGS A C 18 SPE cartridge was washed with 15ml of methanol followed by 5ml of waters. The extract was passed through the column, packed with C 18 sorbent at a flow rate of 1.5ml/min. The column was washed with 20ml of water. The cephalosporin group antibiotics were eluted with 4ml of acetonitrile: water (3:1 v/v). The elute was evaporated to dryness at 45-50 ° C under a stream of nitrogen. The dried residue is reconstituted by addition of 1000µL water and filtered through a 0.45µm PVDF filter. Now the filtrate could be used for qualitative and quantitative analysis. The