How to Cite: Saleh, R. H., Mohammed, H. Q., & Al-badri, S. A. F. A.- badri. (2022). Comparative study of IL- 2, IL- 6 levels for recovered COVID-19 patients and vaccinated participants. International Journal of Health Sciences, 6(S1), 13274–13283. https://doi.org/10.53730/ijhs.v6nS1.8319 International Journal of Health Sciences ISSN 2550-6978 E-ISSN 2550-696X © 2022. Manuscript submitted: 18 March 2022, Manuscript revised: 9 April 2022, Accepted for publication: 27 May 2022 13274 Comparative study of IL- 2, IL- 6 levels for recovered COVID-19 patients and vaccinated participants Rana Hamad Saleh Department of Medical Microbiology, Faculty of Medicine, University of Wasit, Iraq. Corresponding author email: ranah101@uowasit.edu.iq Haethem Qassim Mohammed Department of Pathology, Faculty of Medicine, University of Wasit, Iraq. Saad Abid Farhan Al-badri Department of Medicine, Faculty of Medicine, University of Wasit, Iraq. Abstract---SARS-CoV-2 transmission and hospitalization can be reduced primarily by vaccination. BNT162b2-mRNA vaccine induced both humoral and cellular immune responses against spike proteins. The aim of this cross-sectional study conducted in Wasit Governorate/Iraq is to compare IL-2 and IL-6 levels for recovered COVID-19 patients and participants who were vaccinated with BNT162b2 mRNA vaccine, we used systems serology to investigate the effects and benefit of the BNT162b2 mRNA COVID-19 vaccine on the level of the immune response. A total of 160 male and female blood specimens were collected from the various hospitals and vaccination centers throughout the governorate and divided into four groups: 40 healthy control participants, 40 symptomatic recovered COVID-19 patients (after 1-3 months), 40 participants vaccinated with the 1 st dose of BNT162b2 mRNA (after 21 days of 1 st dose), and 40 participants vaccinated with the 2 nd dose of BNT162b2 mRNA (after 20-30 days of 2 nd dose), aged between (18-61) years old, serum cytokines levels were assayed by ELISA method. By comparing the levels of IL-2 and IL-6 in all our studied groups, our findings found that the groups vaccinated with two doses of BNT162b2 mRNA vaccines was recorded the highest response level compared to the symptomatic recovered COVID-19 group. Meaning that there is a clearer cellular and humoral immune response and the BNT162b2 mRNA vaccine contributes to favorable B cell, and T cell responses. Keywords---SARS-CoV-2, BNT162b2 mRNA vaccine, IL-2, IL-6.