Background: A close link between bone fracture and cognitive function in elderly people is suggested, but there are few reports about bone density and the association with the cognitive function. In this study, we examined relationship between bone density and cognitive functions in healthy elderly Japanese. Methods: For 114 independently living community dwellers in Shimane prefecture in Japan aged 57 years or over, a brief dietary history questionnaire (BDHQ), cognitive function tests (the revised Hasegawa’s Demen- tia Scale: HDS-R, Mini-Mental State Examination: MMSE, and Frontal Assessment Battery: FAB), medical examinations with anthropometric, blood biochemical, fatty acid profiles of plasma and erythrocyte plasma membrane, bone density and plasma levels of bone metabolic markers were examined. Results: The mean MMSE score of volunteers was 27, indicating very mild cognitive impairment. “Age and Gender” were used as a confounding factor since it is associated with bone density. Plasma levels of TRAP-5b, a bone sorption marker, had a significantly negative relationship with HDS-R score and bone density. Bone density had a positive correlation with eicosapentaenoic acid and docosahexaenoic acid levels in erythrocyte plasma membrane and exercise-oriented score. When volunteers were divided into two groups (a higher score group, 29.0 6 0.1; a lower score group, 23.4 6 0.8) according to cutoff value of HDS-R score 27, plasma TRAP-5b level was higher in the lower score group than in the higher score group. Conclusions: A decrease in bone density may affect age-related cognitive decline in elderly Japanese. It was suggested that fish intake and exercise were effective for the prevention of the demen- tia due to the bone fracture. P1-260 RELATIONSHIP BETWEEN HELICOBACTER PYLORI INFECTION AND COGNITIVE PERFORMANCE IN ELDERS Ming-Lun Han 1 , Jen-Hau Chen 2 , Jyh-Ming Liou 3 , Ta-Fu Chen 4 , Yen- Ching Chen 5 , 1 Min-Sheng General Hospital, Taoyan, Taiwan; 2 Department of Geriatrics and Gerontology, National Taiwan University, Taipei, Taiwan; 3 Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan; 4 Department of Neurology, National Taiwan University Hospital, Taipei, Taiwan; 5 Institute of Epidemiology and Preventive Medicine, College of Public Health, National Taiwan University, Taipei, Taiwan. Contact e-mail: minglun@ms18.hinet.net Background: Dementia ranked the 6 th leading cause of death in the elders in USA, which has become an important health issue around the world. Mild cognitive impairment (MCI) is a prodromal phase of Alzheimer’s disease (AD), which is the leading subtype of de- mentia. Chronic infection caused by pathogens, e.g., herpes sim- plex virus and Chlamydia pneumonia, have known to induce neuroinflammation and the following cognitive impairment, including AD and MCI. Recently, some studies explored the asso- ciation between Helicobacter pylori infection and cognitive impair- ment. However, their findings were inconsistent. Methods: Six hundred and four participants aged 65 years or older were recruited from the annual Elderly Health Checkup at National Taiwan Uni- versity Hospital from March 2011 to December 2013. Montreal cognitive assessment (MoCA) was used to assess cognition with a score<24 indicates cognitive impairment. Blood sample was collected to determine serum anti- Helicobacter pylori IgG level. Multivariable logistic regression analysis was performed to explore the relationship between serum anti- Helicobacter pylori IgG level and cognitive impairment adjusting for age, sex, educational level, APOE ε4 status, renal function, stroke, and depressive symptoms. Results: Helicobacter pylori seropositivity was 46.6 and 44.0% for cognitive impaired and normal elders, respectively (p < 0.61). Serum anti- Helicobacter pylori IgG level was significantly associ- ated with cognitive impairment in the elders (adjusted odds ratio ¼ 1.09, 95% confidence interval ¼ 1.01-1.18, p ¼ 0.04). As compared with the model including Helicobacter pylori alone (c-statistic of receiver operating curve ¼ 0.54), multivariable regression model adjusting for potential confounders above showed ideal prediction ability (c-statistic¼ 0.78). Conclusions: This study found that previous Helicobacter pylori infection was associated with the risk of cognitive impairment probably via the enhancement of sys- temic inflammation and neurodegeneration. P1-261 WITHDRAWN P1-262 THE MEDITERRANEAN DIET AND PRESERVATION OF BRAIN CONNECTIVITY IN OLDER SUBJECTS C ecilia Samieri 1 , Amandine Pelletier 1 , Christine Barul 1 , Catherine F eart 2 , Catherine Helmer 1 , Charlotte Bernard 3 , Olivier P eriot 3 , Bixente Dilharreguy 3 , Jean-Franc ¸ois Dartigues 4 , Mich ele Allard 3 , Pascale Barberger-Gateau 1 , Gwena€ elle Catheline 3 , 1 INSERM U897 and Universit e de Bordeaux, Bordeaux, France; 2 INSERM U897, Bordeaux, France; 3 CNRS UMR 5287 - INCIA, CHU and Universit e de Bordeaux, Bordeaux, France; 4 Bordeaux University, Bordeaux, France. Contact e-mail: cecilia.samieri@isped.u-bordeaux2.fr Background: The Mediterranean diet (MeDi) has been related to a lower risk of Alzheimer’s disease (AD), yet the underlying mecha- nisms are unknown. We hypothesized that protection against neuro- degeneration would translate into higher grey matter volumes, while vascular pathways would be more specifically reflected by preserved white matter microstructure. Methods: We included 146 participants from the Bordeaux Three-City study not demented when they completed a dietary questionnaire, who underwent a 3-Tesla MRI examination an average 9 years later, including diffu- sion tensor imaging. Adherence to the Meditteranean diet was re- flected by a 9-point score based on higher intakes of vegetables, fruits, legumes, cereals, fish; lower intakes of meats and dairies, moderate alcohol consumption and a higher ratio of monounsatur- ated:saturated fat. Results: In voxel-by-voxel analyses adjusted for age, gender, education and APOEε4 allele carrier status, and controlled for multiple comparisons, adherence to the Mediterra- nean diet was significantly associated with preserved white matter microstructure (ie, lower diffusivities and higher fractional anisot- ropy) in extensive brain areas (P<0.05 familywise-error corrected for multiple comparisons, see Figure); the relation of the Mediter- ranean diet to lower diffusivity values remained virtually Poster Presentations: P1 P454