Eur Urol Suppl 2010;9(6):554 Conclusions: Our observations revealed, that compared to open NSS with standard excision of the tumour, the RFA circumferentially around the tumour using HABIB 4X have some disadvantages. Mainly, the area of ablation is much more extensive, as well as the quantity of damaged (prior intact) renal parenchyma surrounding the tumour enclosed within avascular rim is much bigger. Secondarily, the precise control of the distance between the pyelo-caycaleal complex walls and HABIB 4X needles tips is questionable. Our modifcation of thermoablation in NSS provides the more precise bleeding control within excised tumour bed with accompanying haemostatic suture ligation withdrawn, as well as the decrement of the ablated tissue area. This manoeuvre enables the reduction of normal kidney parenchyma damage caused by haemostatic sutures (Fig.1), what becomes more important in case of patient with only one sufcient kidney or with chronic renal insufciency. In conclusion our results showed that open NSS using the HABIB 4X device is related to extensive kidney parenchyma damage and high risk of urine cutaneo-kidney fstula development. The combination of open NSS with standard excision of tumour and haemostasis of the site of resected tumour controlled with the HABIB 4X device provides the rapid bleeding control with supplementary oncological margin. N83 ChroNIC pulmoNary dIseases are INdepeNdeNt rIsk faCtors for ComplICatIoNs after radICal NephreCtomy Tokgoz H. 1 , Akduman B. 1 , Unal I. 2 , Erol B. 1 , Akyurek E. 1 , Mungan N.A. 1 1 Karaelmas University, School of Medicine, Dept. of Urology, Zonguldak, Turkey, 2 Cukurova University, School of Medicine, Dept. of Biostatistics, Adana, Turkey Introduction & objectives: We aimed to identify the prognostic factors and the new parameters that might predict postoperative complication rates in a radical nephrectomy cohort. We also evaluated predictive value of Charlson’s comorbidity index (CCI) and, correlation of CCI with the Clavien postoperative complication scale (CPCS). material & methods: Perioperative characteristics of 47 patients undergoing radical nephrectomy were recorded. Following items were assessed: Preoperative patient characteristics including age, gender, CCI, American Society of Anesthesiologists (ASA) physical status classifcation system category, renal and hepatic functions, clinical stage, histopathological subtype of the tumor and preoperative co-morbid conditions including diabetes mellitus, hypertension, chronic pulmonary disease, peptic ulcus, renal and hepatic dysfunction. Postoperative complications were defned as death, wound infection, pneumonia, atelectasis, pulmonary emboli, anemia, sepsis, cardiac arrhythmia, myocardial infarction and deep vein thrombosis. In addition, postoperative complications were also graded according to the CPCS. results: Preoperative chronic pulmonary diseases were found to be signifcant risk factors for the development of postoperative complications. Age adjusted odds ratio was 7.112 (95% confdence interval: 1.035-48.885) for chronic pulmonary disease. The mean CCI in patients who developed postoperative complications was 4.49± 1.95, whereas it was 5.75± 2.60 for patients who did not develop any postoperative complication (p=0.138). However, CCI value was signifcantly correlated with CPCS grade (p=0.011, rho value=0.366). Conclusions: Presence of chronic pulmonary disease is a strong predictor of postoperative complications after radical nephrectomy. Patients with higher preoperative CCI scores may have higher postoperative CPCS grades. Additional studies are warranted. committee on ethics in human investigation. Expression of genes for IGFs, IGF receptors and IGFBP-3 were evaluated by QRT-PCR. Analyses were performed by using STATISTICA 8 software. Non parametric U Manna-Whitneya test has been used to compare the results. results: There were no statistically signifcant diferences in IGFs mRNA expression in specimens from cancer and no cancer tissue. High expression of IGFBP-3 mRNA has been observed in all cRCC samples. IGFBP-3 has been not observed in any sample from non tumor kidney. High expression of IGF-II receptor has been observed in all, except 1, samples from non tumor kidney. Expression of IGF-II receptor has been observed only in 4 cRCC specimens. Conclusions: High expression of IGFBP-3 mRNA is characteristic for all cRCC specimens.Expression of IGF-II receptor mRNA is signifcantly higher in non tumor kidney than in cRCC. N81 the ComparIsoN of p16 expressIoN IN papIllary reNal Cell CarCINomas wIth aNd wIthout baCkgrouNd NephrosClerosIs Sperga M. 1 , Lietuvietis V. 1 , Franckevica I. 2 , Eglitis V. 1 , Kleina R. 1 1 Riga Eastern Clinical University Hospital, Dept. of Pathology, Riga, Latvia, 2 Children Clinical University Hospital, Dept. of Pathology, Riga, Latvia Introduction & objectives: The gene CDKN2A of p16 protein is located in 9p21 chromosomal segment. p 16 is tumour suppressor protein which inhibits cyclin depending kinases. The kinases are regulating the cell cycle G1 and synthesis stage (Serrano M.,1973). The mutations and deletions (LOH- los of heterozygoty) of gene CDKN2A is proved in many tumours (Igaki H., 2003). The increased expression of this gene is described also in processes of aging. The proliferation of steam cells is reducing and it saves from risk of malignisation (Krshnamutry I., 2006). p16 is involved in the genesis of renal cell carcinoma and correlates with better prognosis (Stephen O., 2006). There is not so many dates about p16 expression in renal cell carcinomas associated with nephrosclerosis. Objectives is to detect immunohistochemicaly expression of p16 in papillary renal cell carcinomas (PRCC) with severe background nephrosclerosis and to compare with PRCC without background nephrosclerosis. material & methods: We have analyzed renal pathology in surgical and autopsy material from Latvian Oncology centre. We have examined and compared PRCC with severe nephrosclerosis (n=5), PRCC without background nephrosclerosis (n=8), normal kidneys (n=5), nephrosclerotic kidney (n=5) and high grade PRCC (n=3). We evaluated morphological variation of tumor, its grades (Fuhrman), TNM stage. p16 (clone p16INKA4a) was proved with En Vision methods and expressed in %. We used non parametric statistical test- Mann-Whitney for the comparison of our results. The dates were signifcant at p<0,05. Between grades of tumour and p16 expression we evaluated correlation of Spearman. results: We have observed p16 in normal kidney in small amount and mainly in distal tubules. Expression of p16 increased in kidney with severe nephroslcerosis background till 15% of tubular cells. In proximal tubules p 16 increased not so much. There was signifcant diference between distal tubules in groups of kidney with and without nephrosclerosis (p=0,008). Expression of p16 in PRCC with nephrosclerosis was higher than in PRCC without nephrosclerosis (Z=2,137, p=0,033). In high grade PRCC p16 expression was smaller and it was signifcantly diferent with PRCC with nephrosclerosis (Z=2,236, p=0,025). But comparison of PRCC without nephrosclerosis and high grade PRCC showed that he diference was not so signifcant (Z=1,743, p=0,081). There was not signifcant diference between PRCC with nephrosclerosis and distal tubules in nepfrosclerotic kidney (Z=0,647, p=0,518). There was inverse correlation between tumour grades and p16 expression (R=-0,518 (p=0, 05). Conclusions: 1.Nephrosclerosis as aging processes may induce p16 expression in renal tubules. 2.Tumours developing in areas of nephrosclerosis have higher amount of p16 level then PRCC without nephrosclerosis. 3.There is decreased p16 expression in tumors with higher grades of malignancy. N82 a Novel teChNIque of NephroN sparINg surgery usINg habIb 4x bIpolar radIo-frequeNCy devICe: prelImINary results Wyczolkowski M. 1 , Juszczak K. 2 , Rzepecki M. 1 , Klima W. 1 , Drewniak T. 1 , Maciukiewicz P. 1 1 Memorial Rydygier Hospital, Dept. of Urology, Cracow, Poland, 2 Memorial Rydygier Hospital; Jagiellonian University Medical College, Dept. of Urology and Dept. of Pathophysiology, Cracow, Poland Introduction & objectives: The Nephron Sparing Surgery (NSS) created the necessity of unique techniques development which facilitate the optimal hemostasis of renal parenchyma. In this study the usefulness of HABIB 4X device in NSS was evaluated. material & methods: The open NSS was performed in twelve patients divided into 2 groups: I (n=4) – NSS + HABIB 4X; II (n=8) – NSS + bleeding within the site of tumour controlled with the HABIB 4X, without haemostatic suture ligation. results: In all patients from group I the prolonged drainage and wound healing obtained. Additionally, 2 of them developed the urine cutaneo-kidney fstula. The patients from group II revealed no complications and proper wound healing.