_____________________________________________________________________________________________________ *Corresponding author: E-mail: anisshaikh63@gmail.com; Journal of Pharmaceutical Research International 33(47A): 866-872, 2021; Article no.JPRI.75659 ISSN: 2456-9119 (Past name: British Journal of Pharmaceutical Research, Past ISSN: 2231-2919, NLM ID: 101631759) Stability Study of Mucoadhesive Microsphere Containing Nateglinide by Using Biodegradable Polymer Chitosan Varun Dasari 1 , Anis Shaikh 2* , Dharmesh Sisodiya 2 , Tanu Bhargava 2 , Rameshwar Dangi 3 , Sunil Nagwe 4 , Sharuk L. Khan 5 and Falak A. Siddiqui 5 1 Sri Indu Institute of Pharmacy, Hyderabad, India. 2 Institute of Pharmacy, Vikram University, Ujjain, Madhya Pradesh, India. 3 Ideal College of Pharmacy, Palghar, Maharashtra, India. 4 Ajanta Pharma Limited, Mumbai, Maharashtra, India. 5 MUP's College of Pharmacy (B Pharm), Degaon, Risod, Washim, Maharashtra-444504, India. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JPRI/2021/v33i47A33086 Editor(s): (1) Dr. Jongwha Chang, University of Texas, College of Pharmacy, USA. Reviewers: (1) Neelam Jain, Oriental University, India. (2) S. Ashutosh Kumar, Bharat Pharmaceutical Sciences, India. Complete Peer review History: https://www.sdiarticle4.com/review-history/75659 Received 10 August 2021 Accepted 21 October 2021 Published 30 October 2021 ABSTRACT Aim: Stability Study of Mucoadhesive Microsphere Containing Nateglinide by Using Biodegradable Polymer Chitosan Study Design: The investigation of the stability of Nateglindine containing mucoadhesive microsphere by using chitosan was carried out. Methodology: The present study was performed to test the stability of microspheres formulation. The promising formulations were selected for in-vitro stability studies. Formulations were stored in screw-capped, amber color small glass bottles at 4±1°C, 27±2°C, and 42 ± 2°C for 45 days. After 0, 15, 30, and 45 days they were evaluated for the following parameters like particle size, and percent residual drug content. Results: The particle size of the microspheres was found to increase slightly at 4±1°C from initial to 45 days which might be due to the agglomeration of particles. While at a higher temperature that Original Research Article