~ 590 ~    Journal of Pharmacognosy and Phytochemistry 2017; 6(6): 590-593 E-ISSN: 2278-4136 P-ISSN: 2349-8234 JPP 2017; 6(6): 590-593 Received: 29-09-2017 Accepted: 30-10-2017 Hansraj Dhakar M.Sc. Scholar, Department of Plant Pathology, RCA, MPUAT, Udaipur, Rajasthan, India RS Ratnoo Assoc. Professor Department of Plant Pathology, RCA, MPUAT, Udaipur, Rajasthan, India Anita Jat M.Sc. Scholar, Department of Plant Pathology, RCA, MPUAT, Udaipur, Rajasthan, India Correspondence Hansraj Dhakar M.Sc. Scholar, Department of Plant Pathology, RCA, MPUAT, Udaipur, Rajasthan, India Seed transmission nature of pathogenic seed borne myco flora of wheat (Triticum aestivum L. Em. The Ll.) Seed Samples Hansraj Dhakar, RS Ratnoo and Anita Jat Abstract Seed (Chittorgarh and Udaipur) were surveyed. A total of 80 seed samples, 40 of each district, were collected during 2016. Eight wheat seed borne myco flora viz., Alternaria alternata, Aspergillus flavus, A. niger, Curvularia lunata, Fusarium moniliforme, Rhizopus stolonifer, Mucor spp. and Trichoderma viride were detected and isolated from eighty seed samples. Among the seed borne fungi, A. alternate and F. moniliforme produced distinct seed rot and seedling infection symptoms. Keywords: Wheat, Seed Borne Myco Flora, pathogenic 1. Introduction Seeds are regarded as highly effective means for transporting of plant pathogens over long distances. Numerous examples exist in agriculture literature for the international spread of plant diseases as a result of the importation of seeds that were infected or contaminated with pathogens. Seed borne diseases have been found to affect the growth and productivity of crop plants. Any infections agent (bacteria, fungi, nematode, etc.) which is associated with seeds having potential of causing a disease in a seedling or plant, is teemed as seed borne pathogen (Agarwal and Srivastava 1981) [1] . 2. Material and Method Experimental location The experiment was conducted at Department of Plant Pathology, Rajasthan College of Agriculture (RCA) MPUAT, Udaipur during 2016. Sources of experimental materials Seed to plant transmission studies were carried out in vitro and pot culture conditions. Under in vitro condition, seed transmission test was carried out by employing plain agar test tube- seedling symptoms test. To confirm the seed transmission nature of pathogenic seed borne myco flora, randomly selected one hundred discoloured and infected seeds were used. The seedlings were raised in 160 x 16 mm test tubes each containing 10 ml of 1 per cent water agar. The discoloured and diseased seeds were placed over plain agar media @ one seed per test tube. The control was also maintained by inoculating seeds with pure culture of pathogenic test myco flora and with surface sterilized healthy seeds. Test tubes were plugged with a cotton plug and were incubated at 25+2 0 C under alternating 12 h of light with 12 h of dark period. After 10 days, plugs were removed and kept for incubation. Observations on seedling symptoms appearance were recorded 15 days after germination. Similarly, to confirm the seed transmission nature of pathogenic seed borne myco flora in pot culture condition, randomly selected discoloured infected seeds were used and were grown in pots filled with sterilized soil. The separate control with surface sterilized healthy seeds and inoculated seeds with pure culture of pathogenic test myco flora were also maintained. The test myco flora was multiplied in sterilized rice medium (20 gm rice + 10 ml distilled water) in five hundred ml conical flasks by inoculating with 7 days old culture. The inoculated flasks were incubated at 25+2 0 C for 10 days and were shaken every day to avoid clumping. The pots were filled with sterilized soil and inoculated upper 4 cm layer of the soil with culture grown in rice medium. The inoculated pots were kept in pot house for 24 h for proper soil infestation before sowing. The surface sterilized wheat seeds were sown at the rate of 10 seeds per pot. A set of control was also kept with surface sterilized seeds sown in sterilized un inoculated soil.