Int.J.Curr.Microbiol.App.Sci (2020) 9(6): 263-268 263 Original Research Article https://doi.org/10.20546/ijcmas.2020.906.033 Antibiogram of Isolated Organisms in Canine Pyometra E. Niyas*, S. Reshma, G. S. Shravya, C. Jayakumar, R. H. Naveen Kumar, N. Sarika and Anugraha Mercy Easaw College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India-680651 *Corresponding author ABSTRACT Introduction Pyometra, a life-threatening disease of intact adult bitches, is the accumulation of pus within the uterine lumen especially during or immediately following a period of progesterone dominance (Baithalu et al., 2010). The key feature is the hormonal imbalance which occurs during the luteal phase of oestrous cycle when the uterine immunity is low and the contaminating microorganisms dominate over the protective mechanisms of the female reproductive tract (Johnston et al., 2001). The uterus of a dog normally does not harbour any microbes; usually its presence is due to the movement of organisms from the nonsterile vagina through the patent cervix during proestrus and oestrus, which cannot be cleared prior to the luteal phase (Watts et al., 1996). International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 6 (2020) Journal homepage: http://www.ijcmas.com The present study was undertaken with the intention of identifying and characterizing the common bacteria causing canine pyometra. Ten cases confirmed of canine pyometra presented at University Veterinary Hospital, Mannuthy, formed the subjects of this study. Vaginal swabs were collected aseptically for identification of the causative organism. Based on the cultural, morphological and biochemical characterization, the isolates from majority of the cases were identified as Escherichia coli and Staphylococcus. However, a few isolates were identified as Streptococcus, Pseudomonas and Bacillus. On antibiogram, many of these isolates were found to be resistant to most of the commonly used antibiotics except a few, which highlights the significance of antimicrobial resistance. About ninety per cent of the cases were sensitive to ceftriaxone- tazobactam. The present study emphasizes the importance of identifying the bacterial agents involved in pathogenesis of canine pyometra, and the requisite of an empiric antibiotic therapy initially to be confirmed soon after by an antibiotic susceptibility assessment for effective medical management of the uterine disorder. Keywords Canine, Pyometra, Antibiogram, Staphylococcus, Escherichia coli, Streptococcus and Pseudomonas Accepted: 14 May 2020 Available Online: 10 June 2020 Article Info