Research Article Antidiabetic Activity of Aloe vera Leaves Alethia Muñiz-Ramirez , 1 RosaM.Perez, 2 Efren Garcia, 3 andFabiolaE.Garcia 4 1 CONACYT-IPICYT/CIIDZA,CaminoalaPresadeSanJos´e2055,Col.Lomas4Secci´ on,CP78216SanLuisPotos´ ı S.L.P, Mexico 2 Laboratorio de Investigaci´ on de Productos Naturales, Escuela Superior de Ingenier´ ıa Qu´ ımica e Industrias Extractivas, Instituto Polit´ ecnico Nacional, Av. Instituto Polit´ ecnico Nacional S/N, Unidad Profesional Adolfo Lopez Mateos, CP 07708 Ciudad de Mexico, Mexico 3 Laboratorio de Qu´ ımica Supramolecular y Nanociencias, Instituto Polit´ ecnico Nacional, Acueducto S/N, Barrio la laguna Ticom´ an, CP 07340 Ciudad de M´exico, Mexico 4 Facultad de Ciencias Qu´ ımicas, Universidad Aut´ onoma de San Luis Potos´ ı, Av. Dr. Manuel Nava No. 6-Zona Universitaria, CP 78210 San Luis Potos´ ı S.L.P, Mexico CorrespondenceshouldbeaddressedtoAlethiaMuñiz-Ramirez;alethia.muniz@ipicyt.edu.mx Received 19 December 2019; Revised 25 February 2020; Accepted 27 March 2020; Published 25 May 2020 AcademicEditor:RonaldSherman Copyright©2020AlethiaMuñiz-Ramirezetal.isisanopenaccessarticledistributedundertheCreativeCommonsAttribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. isresearchevaluatedthepotentialofusingthemethanolextractof Aloe vera (L.)Burm.f(AVM)topreventtheformationof AGEs by means of the BSA/glucose assay, BSA-methylglyoxal assay, arginine-methylglyoxal assay, fructosamine, Nε-(carbox- ymethyl)lysine(CML),thiolgroups,andcarbonylprotein in vitro.eeffectofAVMwasalsoevaluatedwithregardtoinhibiting the enzymes α-amylase, α-glucosidase,andpancreaticlipase.Forthis,theplantwasdried,ground,andsubsequentlymacerated with methanol. Aloe vera methanol extract (AVM) significantly decreased the formation of AGEs, as well as the formation of fructosamine,CML,andcarbonylprotein.econcentrationof5mg/mlofAVMpresentedthebestresults.AVMsignificantly inhibited the α-amylase and α-glucosidase enzymes. As regards the content of thiol groups, a significant increase was observed duringthefourweeksoftheexperiment.So,wecanconcludethat Aloe vera methanolextractdecreasestheformationofAGEs and may inhibit the increase in postprandial glucose, suggesting that AVM can prevent diabetes complications associated with AGE. 1.Introduction Protein glycation is considered one of the main causes of complications of diabetes, such as vasculopathy, retinopa- thy, nephropathy and neuropathy, cataracts, and chronic kidneydisease[1].eglycationofproteinsbringsaboutthe formation of advanced glycation end products (AGEs), which modify the structure of proteins and alter enzymatic activity[2].AGEsareformedbythenonenzymaticreaction ofthefreeaminogroupofaproteinwiththecarbonylofa sugar or a reducing aldehyde (called the Maillard reaction) [3], resulting in the formation of Schiff bases, subsequently through a series of reactions, and fluorescent compounds suchaspentosidineandnonfluorescentcompoundssuchas carboxymethyl lysine (CML) are formed [4]. Some AGEs, suchasCMLandpentosidine,havebecomebiomarkersfor glycooxidizing damage [5]. erefore, taking into account the pathological implications of glycation, it becomes es- sentialtodiscoverinhibitorsofproteinglycation,whichmay help reduce and/or prevent complications in diabetes mellitus [2]. For centuries, herbal medicines have been widelyusedtotreatawidevarietyofdiseases.Todate,many of these plants are still used as a first alternative to cure certain diseases in developing countries around the world due to the few side effects they present; it has also been reportedthataround20%ofthemedicinesusedthroughout the world come from plants [6]. In this context, there is a growing interest in discovering safe and nontoxic plant sourcestofindalternativeorcomplementarymedicinesthat help the treatment of various chronic diseases such as Hindawi Evidence-Based Complementary and Alternative Medicine Volume 2020, Article ID 6371201, 9 pages https://doi.org/10.1155/2020/6371201