_____________________________________________________________________________________________________ *Corresponding author: Email: odediransamuel40@gmail.com; Int. J. Trop. Dis. Health, vol. 44, no. 5, pp. 14-24, 2023 International Journal of TROPICAL DISEASE & Health Volume 44, Issue 5, Page 14-24, 2023; Article no.IJTDH.97381 ISSN: 2278–1005, NLM ID: 101632866 Investigations on the Antimalarial Properties of Cleistopholis patens (Annonaceae) Leaf Methanol Extract and Its Partitioned Fractions in Mice Funmilayo G. Famuyiwa a , Samuel A. Odediran a* and Oluyemi J. Oladiran a a Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria. Authors’ contributions This work was carried out in collaboration among all authors. Author FGF initiated the study. Authors FGF and SAO designed the study. Authors FGF and OJO did the plant collection, extraction and partitioning of the plant extract. Author SAO supervised the antimalarial assay studies and performed the statistical analysis of the results. Authors FGF and SAO wrote the first draft and processed the full manuscript for publication. All the authors managed the literature searches, read and approved the final manuscript. Article Information DOI: 10.9734/IJTDH/2023/v44i51405 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/97381 Received: 12/01/2023 Accepted: 15/03/2023 Published: 23/03/2023 ABSTRACT Aim: The methanol extract and partitioned fractions of Cleistopholis patens leaf were evaluated for antiplasmodial activities with a view to establishing its antimalarial potential and identifying the most active partitioned fraction. Methods: The leaf was collected, authenticated, air-dried and powdered. The extract, prepared by maceration in methanol was tested (0-800 mg/kg) against P. berghei berghei in mice using Peter’s four-day test. Normal saline and Chloroquine (10 mg/kg) were negative and positive control Original Research Article