Supporting information Sequential protein unfolding through a carbon nanotube pore Zhonghe Xu 1 , Shuang Zhang 1,2 , Jeffrey K. Weber 3 , Binquan Luan 3 , Ruhong Zhou 3,4* , Jingyuan Li 1* 1 Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, P. R. China 2 Department of Physics, Zhejiang University, Hangzhou 310027, P. R. China 3 IBM Thomas J. Watson Research Center, Yorktown Heights, New York 10598, United States 4 Department of Chemistry, Columbia University, New York, New York 10027, United State Figure S1: The system setup of protein unfolding through CNT-based nanopore. Water and ions are not shown for clarity. The protein was initially positioned such that the chosen pulling terminus (i.e. the N-terminus, left; and C-terminus, right) was situated 8 Å above the CNT entrance. After a 5-ns equilibration, the protein terminus (rendered as a sphere) was then threaded into the nanopore via steered molecular dynamics. Electronic Supplementary Material (ESI) for Nanoscale. This journal is © The Royal Society of Chemistry 2016