Vol 14, Issue 12, 2021 Online - 2455-3891 Print - 0974-2441 STUDY OF HEPATOPROTECTIVE ACTIVITY OF STINGLESS BEE PROPOLIS AGAINTS TOXICITY OF DRUGS 1 Department of Food Technology, Faculty of Agric. Industrial Technology, Universitas Padjadjaran, Indonesia. 2 Department of Community Nutrition, Faculty of Human Ecology, IPB University, Indonesia. 3 National Institute of Health Research and Development, Indonesian Ministry of Health Indonesia. Email: mahani2018@unpad.ac.id Received: 23 September 2021, Revised and Accepted: 10 November 2021 ABSTRACT Objective: The aim of this study is to determine the effect of stingless bee propolis supplementation as a hepatoprotector on the prevention of Drug- induced liver injury (DILI) and the effect of healing and restoring nutrition for DILI patients due to drug induction. Methods: The literature review starts from problem identification, library data collection, reading, taking notes, analyzing, and processing the data obtained and then compiling it into a systematic review. Results: The results of a literature study conducted show that propolis has a good hepatoprotective ability against drugs that cause DILI cases such as the anti-tuberculosis, antibiotic, and antipyretic groups as indicated by the serum glutamic pyruvic transferase, serum glutamic oxaloacetic transferase, total bilirubin, glutathione, and superoxide dismutase values that are close to normal values. In addition, propolis supplementation can accelerate the healing and restoration of the nutritional status of DILI patients. All active compounds contained in propolis such as phytochemicals and lipopolysaccharides work to protect the liver from the toxic effects of DILI through antioxidant mechanisms. Conclusion: Overall, the data from this literature study show that the hepatoprotective activity of propolis has the potential to complement drug therapy to reduce hepatotoxic effects and can conclusively beneficial to accelerate the restoration of nutritional status for DILI patients. Keywords: Antioxidant, Drug-induced liver injury, Hepatotoxic Hepatoprotective, Propolis. INTRODUCTION Drug-induced liver injury (DILI) is a diagnosis in patients who have liver damage caused by consuming drugs that are liver damage or hepatotoxic [1]. DILI can be caused by drugs that are hepatotoxic including anti-tubercular drugs (Rifampicin, Isoniazid, and Pyrazinamide) [2-4], nonsteroidal anti-inflammatory drugs (Ibufenac, Acetaminophen) [5], anti-retroviral drugs (Ritonavir and Indinavir) [6], anti-hyperlipidemic drugs (Statins, Atorvastatin) [7,8], anesthetic agents (CCl4, Chloroform) [9], and antibiotics (tetracycline and monocycline) [10]. The DILI study found that antibiotic, antibacterial, and anti-tuberculosis drugs were drugs with high hepatotoxic effects compared to other drugs. Based on the diagnostic criteria for hepatotoxicity and the population studied, the reported incidence of anti-TB hepatotoxicity increased from 2% to 28% [11]. The study by the US DILIN cohort reported 46% cases of anti-TB hepatoxicity [10]. In India, cases of anti-TB hepatotoxicity were reported in 58% and cases of death due to hepatotoxicity of anti-TB drugs reached 9.5% [12]. For this reason, the WHO strongly encourages research with the main aim of finding new drugs to tackle drugs that are more effective and with the lower side effects than existing drugs [13]. At present, supplements derived from natural products, fruits, vegetables, herbs, and spices have many biological properties and have the potential to fight several diseases [14]. One that is starting to be developed a lot is propolis which has many pharmacological effects. Propolis is a resin collected by bees from various plants, which mixes with saliva and various enzymes to produce a new, different resin. Propolis has antibacterial, anti-inflammatory, antiviral and other biological activities such as anti-inflammatory, local anesthetics, hepatoprotections, anti-tumor, and immunostimulants [15]. Animal studies have shown that propolis has an antimicrobial effect [16,17], antiviral [18,19], antifungals [20,21], anti-parasitic [22,23], anti- inflammatory [22], and anti-tumor [24]. Propolis contains antioxidants and has anti-inflammatory, immunoregulatory, bacteriostatic and bactericidal effects [22,25]. Bhadauria et al. [26] suggested that propolis has the potential as a hepatoprotector in chronic liver injury by maintaining its antioxidant activity. METHODS The literature review starts from problem identification, library data collection, reading, taking notes, analyzing, and processing the data obtained and then compiling it into a systematic review. The method is generally divided into two stages; The first stage is the stage related to the data collected, consisting of: Problem identification, determining library sources based on eligibility criteria and inclusion or exclusion criteria, data collection, and sorting based on the suitability of the literature with the topics discussed. The second stage is processing the data that have passed the sorting, consisting of: Data analysis and interpretation and confirmation. Data that have been confirmed will be included in the review, while unconfirmed data will be re-sorted. RESULTS AND DISCUSSION Hepatoprotective activity of propolis Hepatoprotectors are compounds or substances that can protect cells as well as repair damaged liver tissue due to toxic substances [27]. Free radical damage in the body can be overcome with antioxidants. Antioxidant is defined as a substance that can delay, prevent, or eliminate © 2021 The Authors. Published by Innovare Academic Sciences Pvt Ltd. This is an open access article under the CC BY license (http://creativecommons.org/ licenses/by/4.0/) DOI: http://dx.doi.org/10.22159/ajpcr.2021v14i12.43217. Journal homepage: https://innovareacademics.in/journals/index.php/ajpcr Research Article MAHANI 1,2 *, GREGORIUS A O B 1 , ENDAH WULANDARI 1 , AHMAD SULAEMAN 2 , HARDINSYAH 2 , NUNUNG NURJANAH 3