Shaikh Mahammad Azhar Husain et al., Int. J. Res. Pharm. Sci., 2020, 11(4), 8209-8215 OėĎČĎēĆđ AėęĎĈđĊ IēęĊėēĆęĎĔēĆđ JĔĚėēĆđ Ĕċ RĊĘĊĆėĈč Ďē PčĆėĒĆĈĊĚęĎĈĆđ SĈĎĊēĈĊĘ Published by JK Welfare & Pharmascope Foundation Journal Home Page: www.ijrps.com Standardisation of Kaishore Guggul by HPTLC method using E-guggulsterone as marker compound Birendra Shrivastava 1 , Shaikh Mahammad Azhar Husain *1 , Aamer Quazi 2 1 School of Pharmaceutical Sciences, Jaipur National University, Jaipur-302017, Rajasthan, India 2 K.T. Patel College of Pharmacy, Osmanabad- 413501, Maharashtra, India Article History: Received on: 15 Jul 2020 Revised on: 30 Aug 2020 Accepted on: 09 Sep 2020 Keywords: Kaishore guggul, HPTLC, standardization, E-guggulsterone AćĘęėĆĈę Ayurvedic medications are becoming more popular among physicians and patients due to their superior therapeutic value. Guggulsterone is a plant sterol found in guggul resin. Obesity bone fractures, arthritis, inϐlamma- tion, cardiovascular disease, diabetes, hyperlipidemia, atherosclerosis, and osteoarthritis have all been treated with the resin in Ayurveda from ancient times. In this study, a technique for standardising the marker E-guggulsterone was created. The regresion analysis results for the E-guggulsterone calibra- tion plot revealed a linear connection with a correlation coefϐicient of r 2 = 0.9995, indicating that the amount of E-guggulsterone in the samples will be exact and accurate. This approach was proven to be exact, accurate, and resilient and may be used to analyse samples regularly. * Corresponding Author Name: Shaikh Mahammad Azhar Husain Phone: 8793586642 Email: azharhussain77@gmail.com ISSN: 0975-7538 DOI: https://doi.org/10.26452/ijrps.v11i4.1815 Production and Hosted by IJRPS | www.ijrps.com © 2020 | All rights reserved. INTRODUCTION The mukul mirth tree produces guggul (gum gug- gul), a resin. Since 600 B.C., the guggul tree’s resin has been employed in Ayurvedic medicine. This rny tree is native to Western India and has mini- mal leaves. The hyperlipidemic effects of soluble extracts from the resin were scientiϐically investi- gated in 1966. Guggul leoresin was approved for sale as a lipid-lowering agent in India in 1986 (Arya, 1988; Satyavati, 1988). In 1994 Guggul was ϐirst introduced in the Western medical literature (Singh et al., 1994). Kaishore guggul removes toxins from your blood- stream introduced by a dirty environment or a poor diet. It helps your body regulating out by balanc- ing uric acid production. It’s produced using herbal extracts that have anti-inϐlammatory qualities natu- rally. It aids in the healing of wounds and ulcers. It also helps to improve your digestion. The identiϐication of genuine species, the harvest- ing of the highest quality raw materials, the eval- uation of post-harvesting handling, the assessment of intermediates and ϐinished products, and the detection of harmful or toxic ingredients are all important aspects of quality control for herbal medicines (Pulok and Mukherjee, 2006). Chemical ϐingerprinting is an effective method for ensuring the quality of herbal medications (Bhutani, 2000). As per regulatory norms and standard books such as pharmacopoeias, evaluation of microscopic and macroscopic characteristics and chemical proϐil- ing of botanical plants were carried out for stan- dardisation and to ensure the quality of materi- als (Pharmacopoeia Indian Herbal, 2002; British Herbal Medicine Association, 1996). For the quali- tative detection of impurities, thin layer chromatog- raphy (TLC) and high performance thin layer chro- matography (HPTLC) are useful methods. For qual- ity control and standardisation, numerous analytical © International Journal of Research in Pharmaceutical Sciences 8209