chronic CNIT in 7.14 % cases each. In LDRT recipients, 1 and 5–year patient survival was 100% and 91.7%, death–censored graft survival was 100% and 92.3% and serum creatinine (SCr) was 1.2560.44 mg/dL and 1.6460.44 mg/dL respectively. In DDRT recipients, 1 and 5–year patient survival was 78.6% and 66.3%,death–censored graft survival was 100% and 92.9% and SCr was 2.2661.54 mg/dL and 1.8960.52 mg/dL respectively. In DDRT, acute tubular necrosis (ATN) was most common lesion with early GD and chronic ABMR,chronic CNIT, IF/TA were with late GD patients. In LDRT recipients, TCR was most common lesion with early GD and chronic ABMR in late GD patients. Patient and graft survival are acceptable in both groups. CONCLUSIONS: Renal biopsy is gold standard for diagnosis, management and prognosis of allograft dysfunction. In elderly RT recipients, biopsy monitoring and tailor-made immunosuppression should be encouraged. FP102 INVESTIGATING TYPE III COLLAGEN TURNOVER IN THREE MODELS OF ISCHEMIA REPERFUSION INJURY Daniel Rasmussen 1 , Alexandra Møller 1 , Per Mose Nielsen 2 , Casper Kierulf- Lassen 2 , Federica Genovese 1 , Nadja Sparding 1 , Morten Asser Karsdal 1 , Rikke Nørregaard 2 1 Nordic Bioscience, Herlev, Denmark and 2 Aarhus university, Aarhus, Denmark INTRODUCTION: Despite the underlying pathology, renal fibrosis is a hallmark in chronic kidney disease (CKD). One of the main collagens of the renal interstitial matrix is type III collagen (COL III). Ischemia reperfusion injury (IRI) in the allograft increases the risk of acute kidney injury, delayed graft function, and acute and chronic rejection. In the current study we investigated the turnover of COL III in three different rat models of IRI. METHODS: We measured biomarkers of COL III degradation (C3M) and formation (PRO-C3) in urine of animals undergoing unilateral nephrectomy followed by IRI (NxIRI), STZ animals undergoing IRI (STZ-IRI), and controls). The controls in the NxIRI group underwent unilateral nephrectomy (Nx), but not IRI. A model of bilateral IRI (bIRI) was also investigated where animals were followed for 0, 1, 2 and 3 weeks, at which time they were terminated. Biomarkers in urine were either normalized to urinary creatinine or total urinary output. RESULTS: Whereas the Nx group showed no increase in U-C3M levels during the study, U-C3M levels increased significantly three and seven days after ischemia reperfusion injury in the NxIRI group (both p<0.008). Levels of the COL III formation marker in urine (U-PRO-C3) decreased slightly at the time of Nx in the NxIRI animals (p=0.01) but increased markedly at day 3 after IRI (p=0.02). It thus seems that both degradation and formation of COL III increases in this model after IRI, but that the process of degradation is seemingly favored. In the STZ-IRI animals, levels of both U- C3M and U-PRO-C3 increased significantly at day 5 after IRI (p<0.0001). This indicates that there is a transient, balanced increase in the COL III turnover, a process that may replace the injured tissue. In the bIRI animals, levels of U-C3M closely mirrored the fibrogenic response increasing significantly at week 1 and 2 (both p<0.009) and afterwards returning to sham levels at week 3. U-PRO-C3 showed the same trend as U-C3M but did not reach statistical significance. In this model, a lower degree of injury may explain why the COL III formation process only had to be upregulated slightly to replace injured matrix. CONCLUSIONS: In animals, both formation and MMP-mediated degradation of COL III are increased, but only transiently. This effect may be due to an acute response to injury, which does not reflect the setting in humans where there is a progressive shift of collagen formation over degradation with disease progression. Our results suggest that the remodeling observed in rodent models do not perfectly mimic the chronicity of kidney response to injury in humans. Despite this, levels of U-C3M may be used to investigate whether pathological degradation of COL III can be used to assess patients with IRI, such as kidney transplant recipients, and its relationship with outcome. FP103 MEGALIN, CUBILIN AND CLC-5 GLOMERULAR EXPRESSION IN MINIMAL CHANGE DISEASE (MCD) AND FOCAL SEGMENTAL GLOMERULOSCLEROSIS (FSGS) Lisa Gianesello 1 , Monica Ceol 1 , Hern an Trimarchi 2 , Daniela Stringa 1 , Giovanna Priante 1 , Alejandro Iotti 3 , Annalisa Angelini 4 , Ivan Gonzalez-Hoyos 2 , Franca Anglani 1 , Dorella Del Prete 1 1 Department of Medicine, University of Padua, Padua, Italy, 2 Hospital Brit anico, AEB, Argentina, 3 Hospital Brit anico de Buenos Aires, Buenos Aires, Argentina, Argentina and 4 University of Padua, Padua, Italy INTRODUCTION: Nephrotic syndrome is the hallmark of both MCD and FSGS. Albumin uptake process at proximal tubular level is mainly driven by megalin, cubilin, and ClC-5. It was demonstrated the presence of these 3 proteins not only at tubular but also at glomerular level. The close clinical relationship between MCD and FSGS has suggested a shared pathogenesis but there are increasing evidences that makes this less likely. The aims of this project were: 1) to evaluate the expression of megalin, cubilin and ClC- 5 at glomerular level in MCD and FSGS biopsies; 2) to assess the presence of a correlation between protein expression and clinical data. METHODS: Six renal biopsies of patients with FSGS, 8 MCD and 8 control kidneys were immunostained for ClC-5, megalin and cubilin. Signals were quantified by morphometric analysis. Qualitative analysis was performed to identify megalin, cubilin and ClC-5 signal in glomerular cells considering morphology and localization of the positive cells. Proteinuria, serum creatinine and drugs at the time of biopsy were collected for all patients. Statistical analysis was performed using Mann-Whitney U- test and Spearmann’s rank correlation test. p<0.05 was considered as significant. RESULTS: In both MCD and FSGS there was a decrease in ClC-5, megalin and cubilin positive glomeruli respect to controls (ClC-5 CTRL: 88.1%, MCD 11.1%, FSGS 30.8%) (Megalin CTRL: 59.3%, MCD: 24.3%, FSGS 43.1%) (Cubilin CTRL: 80.2%, MCD: 53%, FSGS: 31.1%), but only ClC-5 resulted to be significantly downregulated (p<0.01). Qualitative analysis revealed no differences in the expression of megalin and cubilin in podocytes of MCD and FSGS compared to controls, while ClC-5 was downregulated in these cells (CTRL: 94.6%, MCD: 33%, FSGS: 75%). PECs disclosed a huge decrease in positivity for megalin in MCD vs controls (CTRL: 46.3%, MCD: 10.6%), an increase in cubilin positivity in FSGS vs controls (CTRL: 19.4%, FSGS 30%), and no differences were observed in ClC-5 expression among control, MCD and FSGS PECs. No significant correlations between clinical data and ClC-5, megalin and cubilin expression were found. CONCLUSIONS: Our results demonstrated a different expression pattern of ClC-5, megalin and cubilin between MCD and FSGS at glomerular level, with a different involvement of podocytes and PECs. ClC-5 downregulation in MCD and FSGS respect to controls could be explained by podocytes’ loss, a typical sign of both diseases, and MCD’s podocytes seem to be more compromised. These preliminary data suggest a different expression pattern of ClC-5, megalin and cubilin in podocytes and PECs of MCD and FSGS, further underlining the existence of a difference in these two podocytopathies. FP104 UTILITY OF TRANSYUGULAR RENAL BIOPSY AS AN ALTERNATIVE TO PERCUTANEOUS BIOPSY M onica Bolufer 1 , Maria Jose ´ Soler 2 , Irene Agraz 1 , Clara Garcia-Carro 1 , Juliana Jaramillo 1 , Karla Arredondo 1 , Roxana Bury 1 , Natalia Ramos 1 , M Antonia Azancot 1 , Alejandra Gabald on 1 , Mercedes Perez 1 , Eugenia Espinel 1 , Daniel Seron 1 1 Hospital Vall Hebron, Barcelona, Spain and 2 Hospital Vall Hebron, pi17/00257., Barcelona, Spain INTRODUCTION: Transjugular renal biopsy (BTY)may be an alternative when percutaneous ultrasound is contraindicated.The objective is to describe our experience with BTY in our center in terms of its indication, diagnosis and complications in renal disease patients. METHODS: Retrospective descriptive study of all transjugular renal biopsies performed in our center between 2003 to 2018. The registry of renal biopsies from the Nephrology department and clinical history were reviewed. RESULTS: 56 BTY were performed. 31 men (55.4%),median age of 62 years (IQ 25- 75[52.5-69.5])More than half presented hematuria at the time of biopsy, median Creatinine of 2.69 mg/dL(IQ 25-75[1.7-4.3]) and median proteinuria in 24 hours of 2g (IQ 25-75[0.39-4.9]).The mean systolic blood pressure (SBP) and (DBP) prerenal biopsy were 140and 75mmHg.The most frequent renal biopsy indication was acute renal failure, followed by chronic kidney disease, in 5 cases the nephrotic syndrome.The most frequent BTY indication was the technical impossibility in 13 of 56 cases (including infracostal kidneys, obesity and COPD), alterations in haemostasia (n=6), thrombocytopenia (n=5) and solitary kidney (n=7). 12.5% of the biopsies were hepato-renal. Number of cylinder average of 2.5 +/- 1.2, number of glomeruli per BTY 5.8+/-5.8.Diagnosis was obtained in 2/3 of the renal transyugular biopsies.The most frequent histological diagnoses were IgA nephropathy, membranoproliferative glomerulonephritis and thrombotic microangiopathy.Three major complications were observed: fornix rupture and two transfusion requirements for contrast extravasations and subcapsular hematoma. CONCLUSIONS: In our center, BTY allowed histological diagnosis of nephropathy in 2/3 of patients.Thus, BTY is a useful technique for renal histological in patients with contraindications for percutaneous renal biopsy. FP105 HISTOLOGICAL FINDINGS IN ACUTE INTERSTITIAL NEPHRITIS – A ROLE FOR SCORING NON-FIBROTIC INFLAMMATION Emma Cannon 1 , Alastair Rankin 1 , Keith Gillis 1 , Jana Crosby 1 , Patrick Mark 1 , Colin Geddes 1 , Jonathan Fox 1 , Bruce Mackinnon 1 , Emily Mcquarrie 1 , David Kipgen 1 1 Queen Elizabeth University Hospital, Glasgow, United Kingdom INTRODUCTION: Acute interstitial nephritis (AIN) is a histological diagnosis. However, the clinical significance of common histological findings is uncertain. i76 | Abstracts Abstracts Nephrology Dialysis Transplantation Downloaded from https://academic.oup.com/ndt/article/34/Supplement_1/gfz106.FP104/5516285 by guest on 26 December 2022