1 Title: Sequential Coupling of Enzymatic Hydrolysis and Fermentation Platform for High Yield and Economical Production of 2,3-Butanediol from Cassava by Metabolically Engineered Klebsiella oxytoca Short title: High Yield and Economical Production of 2,3-BD by engineered K. oxytoca from Cassava Authors: Panwana Khunnonkwao 1 , Sirima Suvarnakuta Jantama 2 , Kaemwich Jantama 1,* , Claire Joannis-Cassan 3 and Patricia Taillandier 3,* Affiliated Address: 1 Metabolic Engineering Research Unit, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Suranaree sub- district, Muang district, Nakhon Ratchasima 30000, Thailand 2 Division of Biopharmacy, Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Warinchamrap, Ubon Ratchathani 34190, Thailand 3 Laboratoire de Génie Chimique, UMR 5503, Université de Toulouse, CNRS, INPT, UPS, Toulouse, France 31432. *Corresponding author: 1 Professor Kaemwich Jantama, Metabolic Engineering Research Unit, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Suranaree sub-district, Muang district, Nakhon Ratchasima 30000, Thailand. E-mail : kaemwich@sut.ac.th 3 Professor Patricia Taillandier, Université de Toulouse, INP-ENSIACET, Laboratoire de Génie Chimique, 31432 Toulouse cedex 04, France. E-mail: Patricia.taillandier@ensiacet.fr Accepted Article This article is protected by copyright. All rights reserved. This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1002/jctb.6643