~ 1520 ~ The Pharma Innovation Journal 2023; 12(5): 1520-1527 ISSN (E): 2277-7695 ISSN (P): 2349-8242 NAAS Rating: 5.23 TPI 2023; 12(5): 1520-1527 © 2023 TPI www.thepharmajournal.com Received: 26-03-2023 Accepted: 30-04-2023 Aniket Bhalchandra Gaikwad Department of Food Technology and Nutrition, Lovely Professional University, Phagwara, Punjab, India Ravindra Kumar Tiwari Department of Food Technology and Nutrition, Lovely Professional University, Phagwara, Punjab, India Pintu Choudhary Department of Food Technology and Nutrition, Lovely Professional University, Phagwara, Punjab, India Navjot Kaur Department of Food Technology and Nutrition, Lovely Professional University, Phagwara, Punjab, India Corresponding Author: Aniket Bhalchandra Gaikwad Department of Food Technology and Nutrition, Lovely Professional University, Phagwara, Punjab, India A comprehensive review on papaya seed oil extraction and recent applications in food industry Aniket Bhalchandra Gaikwad, Ravindra Kumar Tiwari, Pintu Choudhary and Navjot Kaur Abstract The study has been carried out to explore papaya seed oil extraction techniques which are the new source of edible oil in the food industry. Given the global trend towards the utilisation of solid waste and agricultural leftovers, oil extraction could give economic value to a large number of seeds that are generally wasted. In conventional extraction technology there are many shortcomings, like high consumption of energy, more consumption of harmful chemicals. These have forced the food and chemical industries to find new separation "green" techniques which typically use less solvent and energy, such as microwave extraction, supercritical fluid extraction, ultrasound extraction, ultrafiltration, flash distillation, the controlled pressure drop process and subcritical water extraction. Separation under extreme or non-classical conditions is currently a dynamically developing area in applied research and industry. The bioactive compounds present in papaya seeds make its essential oil a promising food for therapeutic benefits. There are traditional and non-traditional methods for extracting oil from papaya seeds. Traditional extraction methods such as soxhlet extraction and solvent extraction as well as non- traditional extraction methods such as enzyme-assisted, ultrasound-assisted, microwave-assisted, and supercritical fluid extractions have been studied. Plants are used to extract vegetable oils, which are then employed in a variety of industrial and food products. This review summarizes the methods and technologies that can be used for the efficient extraction of papaya seed oil, along with its nutritional importance and applications in the food sector. Keywords: Carica papaya L, papaya seed oil, extraction 1. Introduction The Carica papaya is a member of the Caricaceae family (Yanty et al., 2014) [26] . It was first cultivated in Central America and now spread throughout the world tropical and subtropical climates. Papaya fruits are valuable both as food, including concentrates and mixed beverages and in traditional medicine. The report states that global papaya production increased from over 13.74 million metric tones in 2019 to just under 14 million metric tones in 2020. Papaya has high nutritional value as well as medicinal value. Its roots, leaves, peels, fruits, and seeds have nutritional and therapeutic properties. Papaya fruit is elongated and club-shaped up to 6 to 20 inches (15-50 cm) long and 4 to 8 inches thick. Weight of papaya fruit is up to 1 to 2 kilograms sometimes much more (Li et al., 2015) [9] . There are 50 types of papaya varieties that have been identified. However, due to uncontrolled pollination, pure breeding of papaya varieties is decreasing (Samaram et al., 2014) [20] . The skin is waxy and fairly tough. In ripening process it becomes light or deep yellow in color. It is juicy, some type quite musky. The numerous spherical, wrinkled black seeds are adhered to the walls of the expansive central chamber. As shown in Fig 1, the papaya fruits have a 60% pulp present, which is useful for juices, jams, jelly and pepin production (Tan et al., 2020) [23] . The papaya seed accounts for approximately 20% of the weight of the fresh fruits, which are agricultural waste but processed by the food industry, they are edible. Overall, the papaya has 12% peel, which is also agricultural waste (Li et al., 2015) [9] . The roots and leaves of papaya can be used as diuretics, anthelmintics, and to cure bilious disorders. It contain proteolytic enzyme that soothes the stomach and aids in digestion its liquid extract has been used to shrinonsils (Natural Product Radiance, n.d.).