Bienzymatic Supramolecular Complex of Catalase Modified with Cyclodextrin- Branched Carboxymethylcellulose and Superoxide Dismutase: Stability and Anti-Inflammatory Properties Aymara Valdivia, Yunel Pe ´rez, Roberto Cao, * Maysa Ban ˜os, Ariel Garcı ´a, Reynaldo Villalonga * Introduction Cu,Zn-superoxide dismutase (SOD, EC 1.15.1.1) has been proposed as potential enzyme drug for several diseases caused by overproduction of reactive oxygen species. However, the pharmacological activity of this scaven- ger enzyme is limited by its rapid clearance through the kidney [1] and inactivation by H 2 O 2 , its own reaction product. [2] Modification of the enzyme with biocompatible Full Paper A bienzymatic supramolecular assembly of CAT and SOD is reported. CAT was chemically glycosilated with CD branched CMC and then associated with SOD modified with 1-adaman- tane carboxylic acid. SOD was remarkably resistant to inactivation by H 2 O 2 and its anti- inflammatory activity was 4.5-fold increased after supramolecular association with the modified CAT form. A. Valdivia, Y. Pe ´rez, A. Garcı ´a, R. Villalonga Center for Enzyme Technology, University of Matanzas, Autopista a Varadero km 3 1/2, Matanzas, C. P. 44740, Cuba Fax: þ53 45 253 101; E-mail: reynaldo.villalonga@umcc.cu R. Cao, M. Ban ˜os Laboratory of Bioinorganic Chemistry, Havana University, Havana 10400, Cuba E-mail: cao@fq.uh.cu 70 Macromol. Biosci. 2007, 7, 70–75 ß 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim DOI: 10.1002/mabi.200600166