Plant Archives Journal home page: www.plantarchives.org Importance of fruits as valuable food resources, attention has been paid in recent years to study their physicochemical properties. Therefore, this investigation was undertaken to measure the physicochemical properties of the sweet orange genotype available in Bangladesh. A total of 8 genotypes including 3 released varieties (BAU Malta-1, BAU Malta-3, and BARI Malta-1) and 5 lines (CS Jain-001, CS Jain-002, CS Jain-003, CS Ram-001, and Variegated Malta) were included in this trial. Maximum average fruit weight (286.00 g), fruit size in terms of length (92.00 mm), and diameter (82.00 mm), mesocarp thickness (10.00 mm), and width of epicarp equatorial area (76.00 mm) was recorded from CS Jain-001 while the maximum number of segment (28.00), the diameter of fruit axis (23.66 mm), juice content (41.44%), titratable acidity (0.99%) and TSS: TA (33.73) was recorded from BAU Malta-3. TSS and juice pH was recorded maximum of 10.21% and 4.48, respectively in BAU Malta-1. Sugar content was found to be as follows, reducing sugar was the maximum in CS Jain-001 (3.50 %) while the highest total sugar (4.68%) was recorded in BAU Malta-1. Variegated Malta produced the maximum (48.45 mg/100 ml juice) ascorbic acid while CS Jain-001 contained maximum (8.35 mg/100 ml juice) ß-carotene. Therefore, considering fruit quality, BAU Malta-3, BARI Malta-1 can be used as fresh fruit. However, considering the fruit size, CS Jain-001 and CS Jain-002 could be used as breeding material to develop a new sweet orange variety with higher yield potential. Keywords: Sweet orange, Citrus sinensis, physical, chemical, and nutritional properties PHYSICAL AND CHEMICAL PROPERTIES OF SWEET ORANGE GENOTYPES AVAILABLE IN BANGLADESH Md. Anwar Hossain Khan 1 *, M.A. Rahim 2 , Mahbub Robbani 3 , Md. Fakhrul Hassan 3 , Mohammad Amdadul Haque 4 and Zakaria Alam 5 1 Regional Agricultural Research Station, BARI, Akbarpur, Moulvibazar-3200, Bangladesh 2 Department of Horticulture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh 3 Department of Horticulture, Patuakhali Science and Technology University, Dumki, Patuakhali-8602, Bangladesh 4 Horticulture Research Centre, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh 5 Tuber Crops Research Centre, Bangladesh Agricultural Research Institute, Gazipur-1701, Bangladesh (Date of Receiving-31-01-2021; Date of Acceptance-14-04-2021) *Email: anwarpgrc@yahoo.com ABSTRACT e-ISSN:2581-6063 (online), ISSN:0972-5210 Plant Archives Volume 21, No 1, 2021 pp. 1458-1465 DOI Url: https://doi.org/10.51470/PLANTARCHIVES.2021.v21.no1.196 INTRODUCTION Sweet orange (Citrus sinensis Osbeck) belongs to the Rutaceae family originated from south-east Asia and is the most widely used species of citrus fruits, but is used around the world as an excellent source of the body’s immune system, Vitamin C, a strong natural antioxidant (Ibrahim & Yusuf, 2015). In Bangladesh, it is commonly known as Malta. So far, Bangladesh Agricultural Research Institute (BARI) has released two varieties (BARI Malta-1 & BARI Malta-2) and Bangladesh Agricultural University (BAU) has also developed three Malta varieties as BAU Malta-1, BAU Malta-2 & BAU Malta-3 and these varieties are getting popular day by day. It contains sufcient amount of folacine, calcium, potassium, thyamine, niacin, magnesium and diferent phytochemicals such as limonosides, synephrine, hesperidine favonoids, polyphenols or pectin. These biologically active substances prevent arteriosclerosis, cancer, kidney stones, stomach ulcers, cholesterol and blood pressure, which contribute to human health (Etebu & Nwauzoma, 2014). Phytochemicals are nutritional and non nutritionally benefcial bioactive compounds (Patil et al., 2009). Their adequate intake reduces chronic heart disease incidence, mortality and cancer (Aune et al., 2017). Oranges are important fruit crops economically, with an annual production of around 89,28 million metric tonnes, world-wide from 4,63 million ha as of 2019 (FAO, 2020). Sweet oranges are produced worldwide on a large scale and its demand is high due to fewer seeds in fruits. Several researchers have performed biochemical profling of sweet orange cultivars and have identifed increased levels of sugars and minerals including potassium, magnesium, calcium and phosphorous (De Moraes Barros et al., 2012; Topuz et al., 2005). Xu et al., (2008) also recorded greater antioxidant capacities in oranges, recommending more suited for juice processing than mandarins, lemon, grapefruit and pummelo. Rootstock (Hussain et al., 2013), cultivars (Bermejo et al., 2011; Cano et al., 2008), genetic factors (Dhuique-Mayer et al., 2009), climate (Marsh et al., 2000) and cultural activities (Lee & Kader, 2000) primarily afect the quality of fruit. Mostly, the people of Bangladesh consume fruit on a seasonal basis; however, papaya, guava and banana grown very limited scale around the year. Present consumption of fruits in Bangladesh is 82 g per day per person which is far below