INTERNATIONAL JOURNAL OF BIOMEDICINE International Journal of Biomedicine 12(2) (2022) 293-298 http://dx.doi.org/10.21103/Article12(2)_OA17 ORIGINAL ARTICLE Experimental Biology Antibacterial Control of an Extremely Low Frequency Electric Field on Escherichia coli Amr A. Abd-Elghany 1,2 ; Ebtesam A. Mohamad 2 ; Fadel M. Ali 2 ; Ahmed M. M. Yousef 1 ; Marwa Samy 2 ; Nawal Ayman 2 ; Seham AbdelFatah 2 ; Eslam Atef 2 ; Mohamed A. El-Sakhawy 3,4 1 Radiology and Medical Imaging Department, College of Applied Medical Sciences, Prince Sattam Bin Abdul-Aziz University, Alkharj, Saudi Arabia 2 Biophysics Department, Faculty of Science, Cairo University,Cairo, Egypt 3 Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Prince Sattam Bin Abdul-Aziz University, Al-Kharj, Saudi Arabia 4 Department of Medicinal and Aromatic Plants, Desert Research Center, Cairo, Egypt Abstract The aim of this study was to investigate the electric feld frequency and the time of exposure that causes maximum inhibition of Escherichia coli (E. coli) growth. Methods and Results: Bacterial suspensions were subjected to an extremely low frequency electric feld (ELFEF) with a 0.1 Hz interval between 0.2 Hz and 0.4 Hz. The bacterial growth was observed through optical density (OD) readings. OD values were taken every hour for four hours to monitor bacterial growth in both exposed and unexposed samples. The antibiotic susceptibility test was done to determine the difference between the susceptibility of both exposed and unexposed bacterial samples. Structural changes in the exposed bacterial samples were monitored by transmission electron microscope (TEM). The bacterial growth curve revealed a highly signifcant growth inhibition after being exposed to 0.2 Hz at 2 hours’ exposure time. E. coli suspension exposed to ELFEF at inhibition frequency 0.2 Hz showed a signifcant increase in susceptibility to antibiotics Kefex, meropenem, and piperacillin-tazobactam. Conclusion:The current data suggest that treating E. coli with 0.2 Hz for 2 hours is an effective, prospective, and novel technique for reducing cellular growth and dramatic alteration in the cell membrane. TEM clarifed the great destruction of the bacteria cell wall. (International Journal of Biomedicine. 2022;12(2):293-298.) Key Words: extremely low frequency electric feld • E. coli • optical density • transmission electron microscope For citation: Abd-Elghany AA, Mohamad EA, Ali FM, Yousef AMM, Samy M, Ayman N, AbdelFatah S, Atef E, El-Sakhawy MA. Antibacterial Control of an Extremely Low Frequency Electric Field on Escherichia coli. International Journal of Biomedicine. 2022;12(2):293-298. doi:10.21103/Article12(2)_OA17 Abbreviations ELFEF, extremely low frequency electric feld; OD, optical density; TEM, transmission electron microscope. Introduction There is an interest in applications of a pulsed electromagnetic feld with different frequencies in the medical feld, such as pain control, enhancing the effcacy of anticancer drugs, cancer cell proliferation and apoptosis, and transdermal delivery of low permeant drugs. (1-4) Previous studies on extremely low frequency electromagnetic waves succeeded in controlling the growth of Ehrlich tumors. (5,6) It was shown that extremely low frequencies from 6 Hz up to 500 Hz radiation can process wound repair, (7,8) skin, and bones. (7-10) Cellular activity of microorganisms has recently been controlled using