© Elsevier Masson SAS. All rights reserved.   184 Archives of Cardiovascular Diseases Supplements (2015) 7, 183-184 Results: Hypertensive subjects compared to normotensive controls, have higher values of TC, LDL and TG (62.11% versus 22.5%, respectively, P=0.02), and lower HDL values (48.3% versus 8.5% respectively, P=0.04). Hypercholes- terolemia was observed in 58% patients, hypertriglyceridemia in 11.5%, and mixed hyperlipidemia in 30.5% patients. TC values were significantly higher in women with unbalanced hypertension (P=0.001). Conclusion: The prevalence of dyslipidemia in our study population increases with hypertension. Screening of these risk factors, promotion of healthy lifestyle, and the institution of therapy is desirable to reduce their mul- tiplicative effects. 0368 Aortic impedance in older subjects: MR and applanation tonometry study by wave intensity analysis Delphine Lambert (1), Paolo Salvi (2), Athanase Benetos (2), Damien Mandry (3), Pierre-Yves Marie (4), Laure Joly (2) (1) Faculté de Médecine, Laboratoire Rigidité et Thrombose, UMR S 1116, Vandoeuvre Les Nancy, France – (2) CHU Nancy, Gériatrie, Van- doeuvre Les Nancy, France – (3) CHU Nancy, Radiologie, Vandoeuvre Les Nancy, France – (4) CHU Nancy, Médecine Nucléaire, Vandoeuvre Les Nancy, France Introduction: Central pressure waveform can be decomposed into pres- sure transmission and reflection waves. Zc, characteristic impedance of aorta can be measured through the recording of pulse pressure and aortic flow sig- nals and reflects structural and functional properties of elastic central and peripheral muscular arteries. Methods: This study proposes a novel method using wave intensity analysis to assess reflected waves by decomposition of magnetic resonance imaging cen- tral aortic flow and central aortic pressure waveform measured by tonometry in 30 older subjects allowing to obtain the aortic characteristic impedance in the time domain and reflection index (IR) and reflection magnitude (MR). Results: Elderly patients were mean aged of 75.25±5.85 years (14 female, 16 male). Mean Zc value was 424.34±94.41 DSC for the whole aging population. We found a strong negative association between Zc and LVET (r=–0.53, p=0.004). We found also a trend of negative association between Zc and aortic diameter, at the aortic root, (r=–0.36, p=0.05), and pressure augmen- tation (r=–0.36, p=0.06), and also with Aix (r = –0.32, p=0.09). Zc was pos- itively associated with PPA (r = 0.41, p=0.02), and also strongly with HR (r= 0.54, p=0.003). Association between RM and IR were positively significant with aorta length measure by MRI, respectively (r=0.41, p=0.03) for RM and (r=0.42, p=0.03) for IR. RM associated positively with thoracic aorta length (r 2 = 0.15, p=0.04) after adjustment to age and height, IR also associated positively with thoracic aorta length (r 2 = 0.16, p=0.04) after adjustment to age and height. Conclusions: This automatic signal treatment of aortic flow and pulse pressure waveform is a good method for assessment of wave reflections in older subjects allowing obtaining reflection magnitude. Aortic characteristic impedance is inversely correlated to left ventricular ejection time and aortic diameter as new insights suggest. 0068 Protective role of nucleotidases against the development of hypertension Charlotte Roy (1), Bertrand Toutain (1), Anne-Laure Guihot (1), Jean Sévigny (2), Daniel Henrion (1), Gilles Kauffenstein (1) (1) Université d’Angers, UMR CNRS 6214 – INSERM 1083 BNMI, Angers, France – (2) Département de microbiologie-infectiologie et d’immunologie, Québec, Canada Hypertension is characterized by a hypertrophic remodeling of big arteries, increased tone in smallest, endothelial dysfunction and accompa- nied by oxidative stress, inflammation and fibrosis. Extracellular nucleo- tides, which are released under cellular stress, promote deleterious pathological responses (vasoconstriction, inflammation, vascular permea- bility) through P2 receptors activation although the contribution of puri- nergic signaling to cardiovascular pathologies remains to be established. Hydrolysis of these molecules is provided by nucleoside triphosphate diphosphohydrolases (NTPDases), especially NTPDase1 (CD39), highly expressed in the arterial wall. Together with ecto-5’nucleotidase (CD73), these enzymes generate vasoprotective adenosine (ADO anti inflammatory, vasodilatory). Using Apyrase (APY, soluble potato nucleotidase) treatment and CD39 deficient (Entpd1-/-) mice, we evaluated the potential benefit of nucleotides hydrolysis in experimental hypertension. After 12 days of AngII (1mg/kg/day) infusion, with or without APY (45U sc, 15U ip every 3 days), the increase in systolic blood pressure (SBP) and the hypertrophic aortic remodeling were significantly reduced in AngII/APY-treated mice compared to AngII-treated mice. Reversely, in Entpd1-/- mice treated with intermediate dose of AngII (0.5mg/kg/day) the increase in SBP was greater than in Entpd1+/+ mice. This was associated with exacerbated hypertro- phic aortic remodeling. Interestingly, RT-qPCR revealed a decreased CD39 expression level in resistance arteries of AngII-treated mice and SHR rats, suggesting a role for the enzyme in hypertension. The role of CD39 as a regulator of arterial tone through the control of P2Y6 receptor activation is likely, although its contribution in the prevention of vascular inflammation remains to be investigated. Consequently, nucleotidases protect against high blood pressure and represent new therapeutic area in the treatment of hypertension. 0347 Diuretic, antihypertensive and antioxidant effect of olea europaea lea- ves extract, in rats Steliana Ghibu (1), Claudiu Morgovan (2), Oliviu Vostinaru (1), Neli Olah (2), Cristina Mogosan (1), Adriana Muresan (3) (1) Université de Médicine et Pharmacie, Pharmacologie, Cluj Napoca, Roumanie – (2) “Vasile Goldis” Université de l’Ouest, Science Pharma- ceutique, Arad, Roumanie – (3) Université de Médicine et Pharmacie, Physiologie, Cluj Napoca, Roumanie Introduction: Although, there are known the beneficial effects of the fruits and the olive oil, lately a special attention was given to extracts obtained from olive leaves. The studies have reported that oleuropein (the main active com- ponent of olive leaves) has antioxidant, vasodilator, anti-inflammatory proper- ties. Methods: 30 male Sprague-Dawley rats were randomized in 3 groups (n=10) that received orally for seven days: 1. distillate water (control group), 2. Furosemide (10mg/kg), and 3. Leaves extract of Olea europaea (OE). After the first and last dose of vegetal extract, the diuresis and saluresis (urinary Na + and K + concentration) were determined. At the end of the study the systolic blood pressure was recorded, by a non-invasive method. Human erythrocytes were subjected to an oxidative stress induced by 2,2’-azobis (2-amidinopro- pane) hydrochloride (AAPH) in the presence or absence of increasing concen- trations of OE, lipoic acid and trolox (the last two substances were used like reference antioxidants). Results: Furosemide and OE leaves extract induced a significant (p<0.05) increase in rats diuresis and saluresis as compared to control group. For OE extract, the same effect intensity was recorded after the first and the last doses. Also after seven days of treatment, the rats’ systolic blood pressure was sig- nificantly reduced in OE group. In vitro conditions, in the presence of OE extract, extracellular potassium efflux, the signs of erythrocyte membranes early degradation by oxidative stress, was decreased significantly and dose- dependent manner. Conclusions: In our experimental conditions it appears that, OE leaves extract presented diuretic and antihypertensive effects. Also erythrocyte mem- branes are very significantly protected by the antioxidant compound from OE. Our results could open new perspectives for the use of Olea europaea leaves extract like adjuvant therapy in diseases associated with hypertension and oxi- dative stress.