167 April 2023. Volume 17. Number 2 1. Pathobiology Department of Veterinary Faculty, Semnan University, Semnan, Iran 2. Pathobiology Department of Shahmirzad School of Veterinary Medicine, Semnan University, Semnan, Iran 3. Department of Clinical Sciences of Veterinary Faculty, Semnan University, Semnan, Iran Fatemeh Mehrabi 1 , Maryam Rassouli 1,2* , Seyed Hesamodin Emadi Chashmi 3 * Corresponding Author: Maryam Rassouli Address: Pathobiology Department of Veterinary Faculty, Semnan University, Semnan, Iran. E-mail: mrvpar@semnan.ac.ir Research Paper Molecular Detection of Toxoplasma Gondii in Chicken Meats and Eggs in Semnan City, Iran Background: Toxoplasma gondii is a protozoan parasite, a member of the phylum Apicomplexa. Felids are definitive hosts, and all warm-blooded animals and humans are intermediate hosts. The clinical symptoms of toxoplasmosis among chickens are mostly subclinical, but the infection of chickens and eggs is important as a source of protein for human consumption. Objectives: This study aimed to detect T. gondii in chicken meat and egg by molecular examination. Methods: In this study, 100 chicken legs, 50 eggs of free-range hens, and 50 eggs of industrial hens were collected from different stores in Semnan City, Iran. The samples were inspected for the Toxoplasma B1 gene after DNA extraction. Results: According to the results, Toxoplasma DNA was detected in 23% of chicken legs, 36% of eggs of free-range hens, and 20% of eggs of industrial hens. The infection rate was not significantly different between eggs of free-range and industrial hens (P>0.05). Conclusion: Therefore, Toxoplasma is present in chicken meats and eggs in Semnan, Iran, and it is recommended that people eat well-cooked chicken meat and eggs for disease control and feed domestic carnivores with cooked meat to prevent the parasite life cycle. Keywords: Avian, Gallus gallus, Poultry, Prevalence, Toxoplasmosis A B S T R A C T How to Site This Article Mehrabi, F., Rassouli, M., and Emadi Chashmi, S. H. (2022). Molecular Detection of Toxoplasma Gondii in Chicken Meats and Eggs in Semnan City, Iran. Iranian Journal of Veterinary Medicine, 17(1), 167- 172. http://dx.doi.org/10.32598/ijvm.17.2.1005238 http://dx.doi.org/10.32598/ijvm.17.2.1005238 Use your device to scan and read the article online Article info: Received: 01 Jul 2022 Accepted: 15 Sep 2022 Publish: 01 Apr+ 2022