Journal of Experimental Biology and Agricultural Sciences http://www.jebas.org ISSN No. 2320 – 8694 Journal of Experimental Biology and Agricultural Sciences, June - 2022; Volume – 10(3) page 651 – 659 Incidence of Multidrug Resistance Escherichia Coli Bacteria in Broiler Chickens in Malang Regency Fidi Nur Aini Eka Puji Dameanti 1* , Jeffrys Salsabila 1 , HarioPuntodewo Siswanto 1 , Indah Amalia Amri 1 , Sruti Listra Adrenalin 1 , Mira Fatmawati 1 , Taty Aryanti 2 1 Faculty of Veterinary Medicine Universitas Brawijaya, Indonesia 2 Center for Veterinary Research Bogor, Indonesia Received – March 31, 2022; Revision – May 05, 2022; Accepted – June 10, 2022 Available Online – June 26, 2022 DOI: http://dx.doi.org/10.18006/2022.10(3).651.659 ABSTRACT Chicken meat is an important source of protein but the presence of bacterial infections such as colibacillosis is a major concern for the chicken producers. Further, colibacillosis is a major cause of mortality, morbidity, and economic loss for the poultry industry. Various efforts including the use of antibiotics have been carried out to treat colibacillosis. Recently, inappropriate use of antibiotics not only induced antibiotic resistance, but sometimes it might change into multidrug resistance due to a large number of antibiotic uses. This study aimed to identify the incidence of multidrug resistance in broiler chickens on 4 farms of Malang Regency. For this, samples of 40 chicken jejunum swabs that had a history of colibacillosis with clinical symptoms of lethargy, drooping, dwarfism, hair loss, depression, thinness, diarrhea, abdominal swelling, and osteoarthritis were used in this study. Testing begins with a microscopic examination, followed by the isolation of E. coli on Nutrient broth (NB) and Eosin Methylene Blue Agar (EMBA) media, and finally, antibiotic sensitivity was tested against eight antibiotics namely Gentamicin, Bacitracin, Enrofloxacin, Doxycycline, Oxytetracycline, Erythromycin, Colistin, and Amoxicillin on Mueller-Hinton Agar (MHA) media. The microscopic observations showed that the chickens had a hemorrhage in the proventriculus and intestines, pericarditis, and fibrinous exudate in the air sacs and heart. Among the tested samples, 72.5% (29 samples) were found positive for E. coli. Further, in case of antibiotic resistance, 100% of E. coli positive samples were found resistant to Erythromycin, Bacitracin, and Amoxicillin, 96.6% to Enrofloxacin, 92.6% to Oxytetracycline, 37.9% to Colistin and Doxycycline, 10.3% to Gentamicin. Results of the study can be concluded that most of the E.coli positive samples have antibiotic resistance and the maximum samples are showing multidrug resistance against four or more antibiotics. * Corresponding author KEYWORDS Broiler Chicken Escherichia coli Malang Regency Antibiotic Sensitivity Test Multidrug Resistance E-mail: drhfidi@ub.ac.id (Fidi Nur Aini Eka Puji Dameanti) Peer review under responsibility of Journal of Experimental Biology and Agricultural Sciences. All the articles published by Journal of Experimental Biology and Agricultural Sciences are licensed under a Creative Commons Attribution-NonCommercial 4.0 International License Based on a work at www.jebas.org. Production and Hosting by Horizon Publisher India [HPI] (http://www.horizonpublisherindia.in/). All rights reserved.