International Journal of Current Science Research and Review ISSN: 2581-8341 Volume 05 Issue 04 April 2022 DOI: 10.47191/ijcsrr/V5-i4-34, Impact Factor: 5.995 IJCSRR @ 2022 www.ijcsrr.org 1116 * Corresponding Author: Titik Taufikurohmah Volume 05 Issue 04 April 2022 Available at: ijcsrr.org Page No.-1116-1125 The Effect of Nanogold-Nanosilver Injection on Increasing the Immunity of Community Affected by Covid-19 Filza Qurrota ‘Aini 1 , Titik Taufikurohmah 2 1,2 Department of Chemistry, Faculty Mathematics and Natural Sciences, Universitas Negeri Surabaya, Indonesia ABSTRACT: This study aims to determine the best concentration of Nanogold and the effect of Nanogold-Nanosilver injection on increasing the immune system of people affected by Covid-19. The method used is quantitative and quantitative descriptive by observing the progress of giving injections once a month (May – November 2021) regarding the complaints experienced. The best concentration of Nanogold for injection observed on the antioxidant activity and λ maximum stability of Nanogold solution 5, 10, 15, 20, 25, 30 ppm after synthesis and after being stored for 7 days using UV-Vis Spectrophotometry instruments. Produce antioxidant activity 44,21%; 47,88%; 51,37%; 52,32%; 56,16%; 61,57%, with an IC50 of 14 ppm and a λ maximum stability of 5 - 20 ppm is stable; 25 - 30 ppm shifted by 0,50 nm. So, the best concentration of Nanogold was 20 ppm which was then characterized using the TEM instrument, resulting in an average size of 22,67 nm. A mixture of Nanogold-Nanosilver was injected into the respondents and obtained interview data after each injection. Respondents acknowledged that there was a significant difference, as evidenced by the percentage of development of body condition getting better. Thus, Nanogold and Nanosilver materials can increase immunity during the Covid-19 pandemic. KEYWORDS: Covid-19, Immune, Injection, Nanogold, Nanosilver INTRODUCTION Covid-19 is the worst pandemic disease in millennials [1], so it was designated as a global health emergency or PHEIC on March 11, 2020 [2,3]. Coronavirus is a positive-sense RNA virus whose surface is covered with spike-like protrusions ranging from 60 nm to 140 nm in diameter so that it looks like a crown [4]. So that, in the current era, it is needed to use vaccines. Besides that also need drugs, vitamins, supplements, and additional food to strengthen health, especially immunity [14]. Among them, are Nanogold and Nanosilver-based nanoparticles which have been widely used in various medical application [7]. Because, nanoparticles are the current material that is often considered by researchers [5], which are particles with a size between 1-100 nm [6]. To improve the physical, mechanical, and chemical properties without damaging the atomic structure. And will be more reactive because it has a greater opportunity to interact with other materials [5]. Nanoparticles that play a role in increasing immunity include gold nanoparticles (Nanogold) and silver nanoparticles (Nanosilver) which have been widely used in various medical applications [6]. Nanogold plays a role in drug delivery systems. Its antioxidant activity is 10 times greater than that of vitamin E and has been used in pharmaceutical products including drugs [8]. Nanogold works to increase the immune system at the cellular level [9]. Its toxicity tests in vitro and in vivo explain that Nanogold is safe to use in the body [10]. While Nanosilver has been widely used in agriculture and medicine which have antibacterial, antifungal, antiviral, anti-inflammatory, antiangiogenic, and anti-cancer properties [11,12]. Morphological properties of a nanoparticle can be known in terms of shape and size which were analyzed using FE-SEM and TEM (Transmission Electron Microscopy) microscopic techniques [13]. There are many procedures for administering drugs or vitamins into the body, one of which is often used, namely through intramuscular injection [15]. These procedures are usually used for therapy, vaccines, immune suppression, vitamins, antibiotics, and so on. The procedure is carried out by inserting a needle through the skin surface until it enters the muscle layer to increase the drug absorption and bioavailability compared to oral and other procedures [16]. The combination of the two nanoparticles can inhibit viruses from reaching host cell surface receptors because they can help fight infection and develop antibodies in the body [5]. Thus, this study aims to determine the best concentration of .tNanogold to be used and to determine the effect of administering Nanogold-Nanosilver injections on increasing the immunity of people affected by Covid-19.