1 Computational and experimental studies of the breathing motion of a protein loop: implications in PfAMA1-PfRON2 late-stage binding event Suman Sinha, ‡ Anamika Biswas, ‡ Jagannath Mondal* and Kalyaneswar Mandal* Tata Institute of Fundamental Research Hyderabad, 36/p Gopanpally, Hyderabad, Telangana- 500046, India. KEYWORDS Protein-protein interactions, Peptides, Molecular dynamics simulation, Umbrella sampling, Malaria. (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint this version posted February 28, 2022. ; https://doi.org/10.1101/2021.10.10.463826 doi: bioRxiv preprint