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ISSN 0026-2617, Microbiology, 2016, Vol. 85, No. 1, pp. 47–55. © Pleiades Publishing, Ltd., 2016.
Determination of Endoglucanase Activity of Paper Decaying Fungi
from an Old Library at the Ancient Medina of Fez
1
F. El Bergadi
a
, F. Laachari
a
, M. Sadiki
a
, S. Elabed
a, b
, M. H. Iraqui
a
, and S. K. Ibnsouda
a, b, 2
a
Microbial Biotechnology Laboratory, SMBA University, Faculty of Sciences and Technology, Fez, Morocco
b
City of Innovation, SMBA University, Faculty of Sciences and Technology, Fez, Morocco
Received March 2, 2015
Abstract—Paper from an ancient library of the cultural city of Fez (Morocco) is exposed to rapid deterioration
by variety of microorganisms, especially cellulolytic fungi. For this, ten isolates fungi previously isolated from
historical biodeteriorated paper were screened for their ability to produce endoglucanase (CMCase), amy-
lase, polygalacturonase and ligninase enzymes. The CMCase activity of cellulolytic strains was essayed in liq-
uid media at 25°C for 10 days. Influence of temperature and pH were assessed for the production of CMCase
by all the fungus isolated from decaying paper. The research findings from the present study demonstrate that
all the tested isolates had cellulase, amylase, pectinase and ligninase activities. It was found that Mucor race-
mosus PF15, Aspergillus niger, and Aspergillus oryzae exhibited the maximum endoglucanase activity in liquid
medium (0.256, 0.236, and 0.216 UI/mL in descending order) for six days. Temperature profiling revealed
optimum endoglucanase activity at 25 and 30°C. Maximum activity was observed at pH 5 and pH 6.
Keywords: Fez, cellulolytic fungi, carboxymethyl cellulase, remazol brillant blue R
DOI: 10.1134/S0026261716010021
Library documents of the city of Fez represent an
invaluable treasure of Morocco. They are generally
composites of different organic materials (papyrus,
parchments, leather, wood, etc.), each with different
possible responses to environnemental changes. How-
ever, the paper is used frequently as supported materi-
als of these manuscripts and books and it is too
extremely vulnerable to a different external and inter-
nal factors.
Paper is made from pulp which in turn is made
from either wood or other lignocellulosic materials
(Gutleben et al., 2004). It is manly constituted of cel-
lulose (Strzelczyk, 2004; Kacik et al., 2009) that is
readily biodegraded by organisms (Puls et al., 2011). In
fact, the paper can suffers from biodeterioration
because its organic nature. A great variety of fungi and
bacteria can accelerate the biodeterioration of written
or printed paper with historical or economic value for
humans (Cappitelli and Sorlini, 2005). Although, the
fungi are the most important microbial agents (Zyska,
2002; Adelantado et al., 2005), they tolerate desicca-
tion, high salt concentrations, and heavy metal com-
pounds that are present in inks and pigments and are
thus frequent inhabitants on paper supported docu-
ments. Moreover, they are known to produce many
types of enzyme, with cellulases playing a major role in
their deteriorative activity as shown by numerous of
authors (Fabbri et al., 1997; Rojas et al., 2009; Dalal
et al., 2011).
Indeed, fungi may serve as good producers of cel-
lulase. For the complete hydrolysis of cellulose, three
types of cellulases based on their mode of action work
together: endoglucanases or carboxymethyl cellulases
(endo-1,4-glucanase, EC 3.2.1.4) bring out the hydro-
lysis of internal glycosidic bonds in cellulose chains,
creating more terminal ends in the fragments, exoglu-
canases or cellobiohydrolases (exo-1, 4-glucanase,
EC 3.2.1.74) liberate glucose or cellobiose from ends
of cellulose chains due to hydrolysis of terminal glyco-
sidic bonds and β-1,4-glucosidases (β-D-glucohydro-
lases, EC 3.2.1.21) further hydrolyze cellobiose formed
as a result of synergistic action of the above two
enzymes (Jarvis, 2003; Chandra et al., 2007).
Fungi developing on historical paper at elevated
humidity not only actively hydrolyze the cellulose but
may also sporulate abundantly and change in the doc-
uments aesthetics, either by discoloration by weak
acids or by the accumulation of pigments that may
stain its support in a phenomenon referred to as foxing
(Arai, 2000). Fungal deterioration of different paper
materials are well documented phenomena
(Michaelsen et al., 2006; Green et al., 2008; Strlic
et al., 2011; Pinheiro et al., 2011). Most commonly
genera of fungi are found on biodeteriorated paper
(Montemartini Corte and Zotti, 2005), however, cel-
lulolytic fungi are particularly considered as serious
degrading agents of bibliographic documents (Fabbri
1
The article is published in the original.
2
Corresponding author ( e-mail: Ibnsoudas@yahoo.fr).
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