Galore International Journal of Health Sciences and Research DOI: https://doi.org/10.52403/gijhsr.20210404 Vol.6; Issue: 2; April-June 2021 Website: www.gijhsr.com Original Research Article P-ISSN: 2456-9321 Galore International Journal of Health Sciences and Research (www.gijhsr.com) 25 Vol.6; Issue: 2; April-June 2021 Dengue Viral Augmentation among the Population of Few Districts of Assam and Neighbourhood Visiting Tezpur Medical College & Hospital Samrat Biswas 1 , Jyoti Hazarika 2 , Mihirjyoti Pathak 3 , Kalpana Bezborah 4 , Sonuwara Begum 5 , Kaushik Das 6 1 Associate Professor, Department of Microbiology, Tezpur Medical College & Hospital, Tezpur, Assam-784010. 2 Associate Professor of Microbiology, LGB Regional Institute of Mental Health, Tezpur, Assam-784001. 3 Research Scientist-I (Non-Medical), Viral Research & Diagnostic Laboratory, Department of Microbiology, Tezpur Medical College & Hospital, Tezpur, Assam-784010 4 Professor & HOD, Department of Microbiology, Tezpur Medical College & Hospital, Tezpur, Assam-784010. 5 Research Scientist- I (Medical), Viral Research & Diagnostic Laboratory, Department of Microbiology, Tezpur Medical College & Hospital, Tezpur, Assam-784010 6 Research Assistant, Viral Research & Diagnostic Laboratory, Department of Microbiology, Tezpur Medical College & Hospital, Tezpur, Assam-784010. Corresponding Author: Mihirjyoti Pathak ABSTRACT Dengue virus (DENV) has rapidly expanded its range through tropical and subtropical regions in recent years. This pathogen causes acute febrile illness (dengue fever, DF) and severe bleeding disease (dengue hemorrhagic fever, DHF) in humans. In this study we are trying to analyze the data obtained from the diagnosis conducted upon the Dengue suspected patients visiting tertiary care hospital, Sonitpur, Assam and to give a scientific evaluation to the pattern or spread of this epidemic disease. A total of 361 serum samples are analysed from suspected dengue cases during this study period. This study basically gives our research team to go more investigating the dengue viral expansion in coming days as we were able to discriminate among the patients on the basis of primary and secondary infections, what they were previously not examined for. Significant clue for the presence of secondary dengue viral infections (17.3%) among 10 different districts is found. Keywords: Dengue, Viral Research and Diagnostic Laboratory (VRDL), Tezpur Medical College & Hospital (TMCH) INTRODUCTION “It's in the misery of some unnamed slum that the next killer virus will emerge” by noticing an worthy quote by former US President Barack Obama, one can call it’s a day when the urban commons of the world has understood that any disaster could be unleashed by a tiny virus reincarnating as an opportunistic giant. But, such giant could be a more than a disaster for the people of country like India whose residents live in sub-urban or rural area as percentage of 66.46 of total population, who are struggling yet to get basic medical facilities and health hygiene till this moment. Due to such health-compromising situations only, the residents of this developing country repeatedly face the wrath of viral diseases like Dengue, previously in certain months, now almost for the whole year. Dengue virus (DENV) has rapidly expanded its range through tropical and subtropical regions in recent years. This pathogen causes acute febrile illness (dengue fever, DF) and severe bleeding disease (dengue hemorrhagic fever, DHF) in humans. DF and DHF, with an estimated annual infection rate of 100 million and